Skp2和p27kip1蛋白在病理性瘢痕组织中的表达和意义

目的研究Skp2（S期激酶相关蛋白2）在病理性瘢痕中的表达情况及其与p27^kip1之间的相互关系，探讨它们在瘢痕形成中的作用及机制。方法应用免疫组化SP法检测正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中Skp2、p27^kip1蛋白的表达并进行统计学分析。结果病理性瘢痕组织中Skp2蛋白表达增高，与正常皮肤、成熟瘢痕对照组比较差异有统计学意义（P〈0．01）；病理性瘢痕组织中p27^kip1蛋白表达显著低于正常皮肤、成熟瘢痕（P〈0．05）；病理性瘢痕组织中Skp2蛋白和p27^kip1蛋白呈负相关（P〈0．01）。结论Skp2在病理性瘢痕组织中表达增高，可能通过调节p27^kip1等细胞周期调控因子的降解而促进瘢痕组织中细胞的增生，对病理性瘢痕的形成可能起着重要作用。

Expression and significance of Skp2 and p27kip1 protein in pathological scar

ObjectiveTo study the expression of Skp2 gene (s-phase kinase associated protein 2) in the pathological scars and its relationship with p27 kip1 level and to investigate its role and its probable mechanism in the pathogenesis of abnormal scars. Methods Immunohistochemiscal techuique was performered to detect the expression and distribution of Skp2 and p27 kip1 protein in hypertrophic scar (42 cases), keloid (18 cases), normal scar (40 cases) and normal skin (50 cases), statistics was used to analyze the data. Results The positive rate of Skp2 and p27 kip1 protein expression was not statistically defferent between the hypertrophic scar and keloid (P>0.05), while they were all remarkably significant in comparison between normal scar and abnormal scar (P<0.05).In pathological scar the protein of Skp2 and p27 kip1 showed a strong inverse correlation (P<0.01). Conclusion The result indicated that the expression of Skp2 was increased and it may lead to decrease p27 kip1 level in the hypertrophic scar and keloid, Skp2 overexpression might play an important role in the proliferation of fibroblasts and in the pathogenesis of pathological scar by adjusting the regulation of cyclins such as p27 kip1.