|
|||||
|
|
| 纤维化大鼠肝星状细胞IL-10/IL-10R的表达及IL-10干预的影响 | |
| 引用本文： | 张莉娟,郑伟达,黄月红,陈治新,王小众. 纤维化大鼠肝星状细胞IL-10/IL-10R的表达及IL-10干预的影响[J]. 胃肠病学和肝病学杂志, 2010, 19(1) |
| 作者姓名： | 张莉娟 郑伟达 黄月红 陈治新 王小众 |
| 作者单位： | 福建医科大学附属协和医院消化内科,福建,福州,350001 |
| 基金项目： | 福建省高校新世纪创新人才计划项目，福建省教育厅资助项目 |
| 摘 要： | 目的研究实验性肝纤维化大鼠肝星状细胞白介素10(IL-10)/白介素10受体(IL-10R)表达及IL-10干预对其表达的影响。方法60只清洁级SD大鼠,随机分为正常对照(A组)、肝纤维化(B组)和IL-10干预组(C组)。B组和C组以CCl4腹腔内注射构建肝纤维化模型,C组并予IL-10腹腔内注射干预造模。于第7周和第11周分别随机选取一批大鼠,分离肝星状细胞,抽提总RNA,以半定量RT-PCR法检测各组HSC中IL-10和IL-10R mRNA表达情况。结果病理组织学证实肝纤维化模型构建成功,原代HSC分离培养成功,IL-10干预可以减轻其肝纤维化程度。肝纤维化时IL-10和IL-10R的mRNA表达均较正常组有所增强,但随着肝纤维化进展,IL-10的表达逐渐减弱。IL-10干预可以使二者的表达上调,以第11周时变化最为显著。结论大鼠肝HSC可表达IL-10及IL-10R,IL-10可作用于HSC而发挥抗肝纤维化作用。 |
| 关 键 词： | 肝纤维化 肝星状细胞 白介素-10 白介素-10受体 |
Effects of interleukin-10 on expressions of IL-10 and IL-10R mRNA in hepatic stellate cells in liver fibrosis rats |
|
| ZHANG Lijuan,ZHENG Weida,HUANG Yuehong,CHEN Zhixin,WANG Xiaozhong. Effects of interleukin-10 on expressions of IL-10 and IL-10R mRNA in hepatic stellate cells in liver fibrosis rats[J]. Chinese Journal of Gastroenterology and Hepatology, 2010, 19(1) | |
| Authors: | ZHANG Lijuan ZHENG Weida HUANG Yuehong CHEN Zhixin WANG Xiaozhong |
| Affiliation: | ZHANG Lijuan,ZHENG Weida,HUANG Yuehong,CHEN Zhixin,WANG Xiaozhong Department of Gastroenterology,Union Hospital of Fujian Medical University,Fuzhou 350001,China |
| Abstract: | Objective To study the effects of interleukin-10 on expressions of IL-10 and IL-10 receptor(IL-10R)mRNA in hepatic stellate cells in experimental liver fibrosis rats.Methods Sixty SD rats were randomly divided into control group(group A),liver fibrotic group(group B) and IL-10 treated group(group C).Fibrotic group and IL-10 treated group were injected intraperitoneally with carbon tetrachloride(CCl4).IL-10 treated group was given IL-10 intraperitoneally from the third week.Hepatic stellate cells were isolat... |
| Keywords: | Liver fibrosis Hepatic stellate cell Interleukin-10 Interleukin-10 receptor |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |