胃黏膜病变组织中RASAL_1基因的表达及其与幽门螺杆菌感染的关系

目的探讨RASAL1基因在幽门螺杆菌(Hp)感染胃黏膜组织中的变化及其在胃癌发生发展中的意义。方法内镜及活检病理证实的慢性胃炎58例(慢性胃炎组)、肠化生及异型增生58例(肠化生及异型增生组)和胃癌58例(胃癌组),分别用13C-呼气试验及Giemsa染色检测Hp,链霉菌亲生物素蛋白-过氧化物酶连接(SP)免疫组化检测RASAL1,比较各组及组内Hp阳性亚组和Hp阴性亚组中RASAL1的表达。结果慢性胃炎组、肠化生及异型增生组和胃癌组中RASAL1的阳性率分别为89.66%、56.90%和13.79%,呈递减趋势。各组Hp阳性亚组与Hp阴性亚组中RASAL1的阳性率分别为,慢性胃炎组:73.33%和96.43%,肠化生及异型增生组:40.63%和76.92%,胃癌组:14.29%与13.51%,两两比较均有显著差异。结论 Hp感染可降低胃黏膜RASAL1表达,削弱其对胃黏膜损伤的保护作用,促进了胃癌的发生发展。

Expression of RASAL1 in gastric mucosa lesions and its relationship with helicobacter pylori infection

Objective To explore the changes of RASAL1 gene in gastric mucosa tissue infected by helicobacter pylori(Hp) and its significance in the development of gastric cancer.Methods Hp in 58 patients with chronic gastritis confirmed by endoscope and biopsy pathology(chronic gastritis group),58 patients with intestinal metaplasia and dysplasia(intestinal metaplasia and dysplasia group) and 58 patients with gastric cancer(gastric cancer group) was detected by 13C urease breath test and Giemsa stain,respectively.RASAL1 was determined by streptomyces biophile protein-streptavidinbiotinper-oxidase(SP) immunochemiohistology.The expression of RASAL1 in positive and negative Hp subgroups of each group and within groups was compared.Results The positive rates of RASAL1 gene in chronic gastritis group,intestinal metaplasia and dysplasia group and gastric cancer group were 89.66%,56.90% and 13.79%,respectively,with a decline trend.The positive rates of RASAL1 in positive and negative Hp subgroups of each group were respectively 73.33% and 96.43(chronic gastritis group),40.63% and 76.92%(intestinal metaplasia and dysplasia group) as well as 14.29% and 13.51%(gastric cancer group).Significant differences were observed within groups.Conclusion Hp infection can decrease the expression of RASAL1 in gastric mucosa,alleviate its protective effect on gastric mucosa and promote the occurrence and development of gastric cancer.