非小细胞肺癌中RECK基因的表达及其与MMP-9的关系

目的：探讨非小细胞肺癌组织中RECK和MMP-9蛋白的表达和相关性。方法：采用免疫组化SP法检测RECK和MMP-9在48例肺癌组织和癌旁正常肺组织中的表达情况。结果：肺癌组织中RECK表达阳性率为29．2％（14／48），癌旁正常肺组织中阳性表达率为85．7％（42／48），两组比较差异有统计学意义（P〈0．05）。肺癌组织中MMP-9表达阳性率为68．8％（33／48），癌旁正常肺组织中阳性表达率为16．7％（33／48），两组比较差异有统计学意义（P〈0．05）。RECK和MMP-9的表达与淋巴结转移和TNM分期有关，而与患者年龄、性别、肿瘤大小、组织学分化程度无关。与无淋巴结转移和Ⅰ、Ⅱ期的肺癌组织相比，在有淋巴结转移和Ⅲ期的肺癌组织中，RECK表达降低，而MMP-9表达增高（P〈0．05）。RECK和MMP-9的表达经x2检验有负相关关系（χ^2=8．263，r=-0．437，P〈0．05）。结论：RECK蛋白在肺癌中低表达，MMP-9蛋白高表达，二者可能参与了肺癌的发生，在肺癌的侵袭和转移中发挥重要作用，并且具有协同作用。

Study on the expression and correlation of RECK and MMP -9 in non -small cell lung cancer

Objective： To study the expression and correlation of RECK and MMP- 9 in non -small cell lung cancer（ NSCLC） and clinic pathological features. Methods： Immunohistochemical SP method was used to detect the expression of RECK and MMP -9 proteins in 48 NSCLC tissues and the para -cancerous tissues. Results：The positive rate of RECK in NSCLC carcinoma tissues was 29.2% （ 14/48 ） lower than that in para -cancerous tissues 85.7% （42/48） （ P 〈 0.05 ）. Positive rates of MMP - 9 were 68.8% （ 33/48 ） and 16.7% （ 33/48 ） respetively. The positive rates of RECK and MMP -9 were correlated with lymph node metastasis and TNM stage, but no relationship with age, sex, pathology subtype and histological grades. The positive rates between RECK and MMP- 9 were associated （r = -0. 437, P 〈 0.05 ）. Conclusion：RECK is lower - expressed in NSCLC, while MMP -9 is over - expressed. They may be involved in the carcinogenesis of NSCLC, and may play an important role in invasion and metastasis of NSCLC. RECK and MMP -9 play a cooperative role in the process of NSCLC.