扩展流式细胞术的临床应用范围提高应用水平

随着多参数、高通量等分析技术的不断改进,FCM作为表型分析的重要技术平台已逐渐扩展到蛋白质功能分析,针吸活检细胞、脑脊髓液、循环肿瘤细胞以及微生物的检测等临床应用领域.目前,流式标本处理、仪器设置以及数据分析等是制约FCM在临床检验常规应用的主要问题,因此应制定规范统一的流式项目标准操作流程,并建立地区性的流式共享平台,使FCM更好地服务于临床检验.

Abstract：

With the improvement of the multi-parameter and high throughput technology,FCM has expanded its clinical application from cellular phenotying to other areas,such as protein function analysis,fine needle aspiration,cerebrospinal fluid determination,circulating tumor cell detection as well as clinical microbiology.Currently,lack of standards for the sample processing,equipment adjustment and data analysis remains the main obstacle for routine application of FCM.Therefore,it is recommended to establish consensus standard operation procedures as well as shared FCM platform regionally to provide better service of FCM in clinical laboratory.

Extending the clinical application and improving the practical techniques of flow cytometry

With the improvement of the multi-parameter and high throughput technology,FCM has expanded its clinical application from cellular phenotying to other areas,such as protein function analysis,fine needle aspiration,cerebrospinal fluid determination,circulating tumor cell detection as well as clinical microbiology.Currently,lack of standards for the sample processing,equipment adjustment and data analysis remains the main obstacle for routine application of FCM.Therefore,it is recommended to establish consensus standard operation procedures as well as shared FCM platform regionally to provide better service of FCM in clinical laboratory.