| 人牙周膜成纤维细胞对盐酸林可霉素跨膜转运的研究 |
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| 引用本文： | 刘宇,刘洪臣,吴霞,鄂玲玲,冷斌. 人牙周膜成纤维细胞对盐酸林可霉素跨膜转运的研究[J]. 临床口腔医学杂志, 2009, 25(5) |
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| 作者姓名： | 刘宇 刘洪臣 吴霞 鄂玲玲 冷斌 |
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| 作者单位： | 海军总医院口腔科,北京,100037;解放军总医院口腔医学中心,北京,100853;解放军总医院口腔医学中心,北京,100853 |
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| 摘 要： | 目的:研究人牙周膜成纤维细胞(human periodontal ligament fibroblasts,HPDLF)对盐酸林可霉素的跨膜转运,为根管牙周局部及全身给药提供实验依据.方法:盐酸林可霉素溶液孵育HPDLF和MC3T3-E1细胞,超声破碎细胞后,HPLC测定不同时间点的胞内药物含量,考马斯亮蓝法测定细胞蛋白总量.结果:10 μg/ml的盐酸林可霉素孵育HPDLF及MC3T3-E1 细胞 1 min、5 min、10 min时均无法检测出胞内药物的存在,20 μg/ml的药物作用5 min、1O min,同样无法检测.结论:HPLC可以用于盐酸林可霉素的测定.本实验条件下不能检测出HPDLF以及MC3T3-E1细胞对盐酸林可霉素的跨膜转运.
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| 关 键 词： | 人牙周膜成纤维细胞 盐酸林可霉素 生物转运 高效液相色谱法 |
Biological transport of lincomycin hydrochloride by human periodontal ligament fibroblasts |
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| LIU Yu,LIU Hong-chen,WU xia,E Ling-ling,LENG Bin. Biological transport of lincomycin hydrochloride by human periodontal ligament fibroblasts[J]. Journal of Clinical Stomatology, 2009, 25(5) |
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| Authors: | LIU Yu LIU Hong-chen WU xia E Ling-ling LENG Bin |
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| Affiliation: | LIU Yu1,2,LIU Hong -chen2,WU Xia2,E Ling -ling2,LENG Bin2. 1.Department of Dentistry,Navy General Hospital of PLA,Beijing 100037,China,2.Dental Institute,General Hospital of PLA,Beijing 100853,China. |
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| Abstract: | Objective:To investigate biological transport of lincomycin hydrochloride by human periodontal ligament fibroblasts (HPDLF)to verify the hypothesis of delivering medicine to periodontium and the whole body through the root canal. Method:HPDLF and MC3T3-E1 cells were incubated in antibiotics solutions. The intracellular antibiotics contents were measured by HPLC and the cell total protein was measured by Bradford protein assay. Result:The intracellular antibiotics content of HPDLF and MC3T3-E1 cells couldn't... |
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| Keywords: | human periodontal ligament fibroblasts lincomycin hydrochloride biological transport high performance liquid chromatography |
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