| Effects of viral infection and microbial diversity on patients with sepsis:A retrospective study based on metagenomic next-generation sequencing |
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| 作者姓名： | Li-wei Duan Jin-long Qu Jian Wan Yong-hua Xu Yi Shan Li-xue Wu Jin-hao Zheng Wei-wei Jiang Qi-tong Chen Yan Zhu Jian Zhou Wen-bo Yu Lei Pei Xi Song Wen-fang Li Zhao-fen Lin |
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| 作者单位： | Department of Emergency and Critical Care Medicine;Department of Emergency and Critical Care Medicine |
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| 基金项目： | supported by grants from Scienceand Technology Committee of Shanghai (18411951400);KeyClinical Medical Specialties Project in Shanghai Pudong NewArea (PWZzk2017-22);Science and Technology Action Plan(19495810200);Leading Talent Project in Shanghai Pudong NewArea Health System (PWRl2018-08). |
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| 摘 要： | BACKGROUND: The study aims to investigate the performance of a metagenomic next-generationsequencing (NGS)-based diagnostic technique for the identifi cation of potential bacterial and viral infectionsand eff ects of concomitant viral infection on the survival rate of intensive care unit (ICU) sepsis patients.METHODS: A total of 74 ICU patients with sepsis who were admitted to our institution from February1, 2018 to June 30, 2019 were enrolled. Separate blood samples were collected from patients for bloodcultures and metagenomic NGS when the patients’ body temperature was higher than 38 °C. Patients’demographic data, including gender, age, ICU duration, ICU scores, and laboratory results, were recorded.The correlations between pathogen types and sepsis severity and survival rate were evaluated.RESULTS: NGS produced higher positive results (105 of 118;88.98%) than blood cultures(18 of 118;15.25%) over the whole study period. Concomitant viral infection correlated closelywith sepsis severity and had the negative effect on the survival of patients with sepsis. However,correlation analysis indicated that the bacterial variety did not correlate with the severity of sepsis.CONCLUSIONS: Concurrent viral load correlates closely with the severity of sepsis and thesurvival rate of the ICU sepsis patients. This suggests that prophylactic administration of antiviraldrugs combined with antibiotics may be benefi cial to ICU sepsis patients.
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| 关 键 词： | SEPSIS Metagenomic next-generation sequencing Viral infections Bacterial infections Microbial diversity |
| 收稿时间： | 2020-01-15 |
Effects of viral infection and microbial diversity on patients with sepsis: A retrospective study based on metagenomic next-generation sequencing |
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| Authors: | Li-wei Duan Jin-long Qu Jian Wan Yong-hua Xu Yi Shan Li-xue Wu Jin-hao Zheng Wei-wei Jiang Qi-tong Chen Yan Zhu Jian Zhou Wen-bo Yu Lei Pei Xi Song Wen-fang Li Zhao-fen Lin |
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| Institution: | Department of Emergency and Critical Care Medicine, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China
Department of Emergency and Critical Care Medicine, Pudong New Area People’s Hospital, Shanghai 201299, China |
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| Abstract: | BACKGROUND: The study aims to investigate the performance of a metagenomic next-generation sequencing (NGS)-based diagnostic technique for the identification of potential bacterial and viral infections and effects of concomitant viral infection on the survival rate of intensive care unit (ICU) sepsis patients. |
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| Keywords: | Sepsis Metagenomic next-generation sequencing Viral infections Bacterial infections Microbial diversity |
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