Effects of different scaffolds on rat adipose tissue derived stroma cells |
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| Affiliation: | 1. Department of Plastic and Reconstructive Surgery (Head: M. Kon), University of Utrecht Medical Center, Heidelberglaan 100, PO Box 85500, 3508 GA Utrecht, The Netherlands;2. Department of Neurosurgery (Head: L.P.E. Regli), University of Utrecht Medical Center, Heidelberglaan 100, PO Box 85500, 3508 GA Utrecht, The Netherlands;3. Department of Oral-Maxillofacial Surgery (Head: R. Koole), University of Utrecht Medical Center, Heidelberglaan 100, PO Box 85500, 3508 GA Utrecht, The Netherlands;1. Oral and Maxillofacial Diseases Research Center, School of Dentistry, Mashhad University of Medical Sciences, Vakilabad Blvd, Mashhad, Iran;2. Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Vakilabad Blvd, P.O. Box: 91735-984, Mashhad, Iran;1. Department of Oral & Maxillofacial Surgery, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden;2. Institute of Surgical Sciences, Department of Oral & Maxillofacial Surgery, Uppsala University, Uppsala, Sweden;1. Department of Oral and Maxillofacial Surgery, University Hospital of Schleswig-Holstein, Campus Kiel, Kiel, Germany;2. Department of Orthodontics, University Hospital of Schleswig-Holstein, Campus Kiel, Kiel, Germany;3. Institute of Medical Psychology and Medical Sociology, University Hospital of Schleswig-Holstein, Campus Kiel, Kiel, Germany;4. Clinic for Child and Adolescent Psychiatry, Johann Wolfgang Goethe-University, Frankfurt, Germany;1. Department of Oral, Maxillary and Plastic Facial Surgery and Interdisciplinary Department of Oral Surgery and Implantology (Head: Prof. Dr. Dr. J.E. Zöller), University of Cologne, Kerpener Str. 62, G-50937 Cologne, Germany;2. Department of Prosthodontics (Head: Prof. Dr. M. Wichmann), University Hospital Erlangen, Glückstr. 11, G-91054 Erlangen, Germany |
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| Abstract: | ![]()
BackgroundAdipose tissue derived stroma cells (ASC's) offer for many advantages for tissue engineering strategies over mesenchymal stroma cells from other sources and ideal carrier materials have to be identified for them. The aim of this study was to demonstrate and to compare the effects of three clinically established biomaterials on proliferation and metabolic activity of rat ASC's in vitro.Materials and methodsRat adipose tissue derived stroma cells (ASC's) were isolated and differentiated into distinct lineages proved by lineage specific staining and gene expression analysis (RT-PCR). The biomaterials Bio-Gide®, Tutodent® Membrane and Belotero® Soft were tested with rat ASC's for their biocompatibility using scanning electron microscopy (SEM), cell vitality staining, cytotoxicity and proliferation tests (LDH, MTT, BrdU, WST-1).ResultsThe collagen membrane Bio-Gide® resulted in a significantly higher viability and proliferation (WST-1, BrdU) compared to Tutodent® Membrane. No significant difference was determined in the LDH and MTT test. The hyaluronic acid gel Belotero® Soft showed no cytotoxicity (LDH, FDA/PI) and had no negative effects on metabolic activity (WST-1, MTT) or cell proliferation (BrdU) of ASC's.ConclusionOur results indicate Bio-Gide® and Belotero® Soft as preferable carrier materials for ASC's. For the further establishment of ASC's-based treatment strategies, in vivo investigations on the tissue regeneration potential of these cell-biomaterial scaffolds should follow. |
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| Keywords: | Adipose tissue derived stroma cells (ASC's) Collagen membranes Hyaluronic acid gel Tissue engineering Multilineage potential Scaffold Biocompatibility |
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