Effect of Emdogain enamel matrix derivative and BMP-2 on the gene expression and mineralized nodule formation of alveolar bone proper-derived stem/progenitor cells |
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| Affiliation: | 1. Clinic for Conservative Dentistry and Periodontology (Head: Prof. Dr. C. Dörfer), School of Dental Medicine, Christian Albrechts-University of Kiel, Arnold-Heller-Str. 3, Haus 26, 24105 Kiel, Germany;2. Oral Medicine and Periodontology Department, Faculty of Oral and Dental Medicine, Cairo University, Egypt;3. Clinic for Applied Cellular Therapy, Christian Albrechts-University of Kiel, Kiel, Germany;4. Department of Clinical Pathology, Faculty of Medicine, Cairo University, Egypt;5. Department of Oral and Maxillofacial Surgery, Christian Albrechts-University of Kiel, Kiel, Germany;6. Department of Operative and Preventive Dentistry, Charité - Universitätsmedizin Berlin, Berlin, Germany;1. Hunan Key Laboratory for Micro-Nano Energy Materials and Devices, Faculty of Materials and Optoelectronic Physics, Xiangtan University, Hunan 411105, PR China;2. Hunan Key Laboratory for Computation and Simulation in Science and Engineering, Faculty of Mathematics and Computational Science, Xiangtan University, Hunan 411105, PR China;3. State Key Lab of Silicon Materials, Zhejiang University, Hangzhou 310027, PR China;1. PG Trainee, Department of Oral and Maxillofacial Surgery, M.S. Ramaiah Dental College and Hospital, Bangalore, Karnataka, India;2. Professor and Department Head, Department of Oral and Maxillofacial Surgery, M.S. Ramaiah Dental College and Hospital, Bangalore, Karnataka, India;3. Senior Professor, Department of Oral and Maxillofacial Surgery, M.S. Ramaiah Dental College and Hospital, Bangalore, Karnataka, India;4. Professor, Department of Oral and Maxillofacial Surgery, M.S. Ramaiah Dental College and Hospital, Bangalore, Karnataka, India;6. Reader, Department of Oral and Maxillofacial Surgery, M.S. Ramaiah Dental College and Hospital, Bangalore, Karnataka, India;5. PG Trainee, Department of Oral and Maxillofacial Surgery, M.S. Ramaiah Dental College and Hospital, Bangalore, Karnataka, India;1. Department of Oral and Maxillofacial Surgery (Head: Mohammad Zandi, DDS, MSc.), Hamedan University of Medical Sciences, Hamedan, Iran;2. Department of Pathology (Head: Alireza Monsef, MD, APCP), Hamedan University of Medical Sciences, Hamedan, Iran;3. Oral and Maxillofacial Surgeon in Private Practice, Tehran, Iran;4. Oral and Maxillofacial Surgeon in Private Practice, Mashhad, Iran |
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| Abstract: | The objective of this study was to evaluate the effect of Emdogain (Enamel Matrix Derivative, EMD) and Bone Morphogenetic Protein-2 (BMP-2), either solely or in combination, on the gene expression and mineralized nodule formation of alveolar bone proper-derived stem/progenitor cells.Stem/progenitor cells were isolated from human alveolar bone proper, magnetically sorted using STRO-1 antibodies, characterized flowcytometrically for their surface markers' expression, and examined for colony formation and multilineage differentiation potential. Subsequently, cells were treated over three weeks with 100 μg/ml Emdogain (EMD-Group), or 100 ng/ml BMP-2 (BMP-Group), or a combination of 100 ng/ml BMP-2 and 100 μg/ml Emdogain (BMP/EMD-Group). Unstimulated stem/progenitor cells (MACS+-Group) and osteoblasts (OB-Group) served as controls. Osteogenic gene expression was analyzed using RTq-PCR after 1, 2 and 3 weeks (N = 3/group). Mineralized nodule formation was evaluated by Alizarin-Red staining.BMP and EMD up-regulated the osteogenic gene expression. The BMP Group showed significantly higher expression of Collagen-I, III, and V, Alkaline phosphatase and Osteonectin compared to MACS+- and OB-Group (p < 0.05; Two-way ANOVA/Bonferroni) with no mineralized nodule formation.Under in-vitro conditions, Emdogain and BMP-2 up-regulate the osteogenic gene expression of stem/progenitor cells. The combination of BMP-2 and Emdogain showed no additive effect and would not be recommended for a combined clinical stimulation. |
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| Keywords: | Stem cells Tissue engineering Alveolar bone proper EMD BMP-2 |
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