HPLC法测定小鼠血浆和组织中伊曲康唑的含量

目的建立高效液相色谱法测定小鼠血浆和组织中伊曲康唑的含量.方法采用色谱柱:Diamosil C18(4.6mm×150mm,5μm);流动相:甲醇-水(84∶16,V/V);流速:1.0mL·min-1;检测波长:262nm;柱温:室温;进样量:20μL,按伊曲康唑峰计理论塔板数不小于3000,来测定小鼠血浆和各组织中伊曲康唑的含量.结果小鼠血浆和各组织器官日内精密度和日间精密度RSD均小于4%;小鼠血浆方法回收率大于97%,RSD均小于3%;小鼠各组织器官方法回收率均大于95%,RSD均小于4%;可满足生物样品分析方法的要求.结论建立的HPLC法测定血浆和组织伊曲康唑的浓度,方法简便,准确,内源性物质不干扰测定,适合于大批量生物样本中伊曲康唑含量的测定.

Establishment of itraconazole determination in plasma and tissue of mice

OBJECTIVE To establish an HPLC method for the determination of itraconazole in mice plasma and tissue.RESULTS The chromatographic column was Diamosil C18（4.6mm×150mm,5μm）;The mobile phase was methanol-water（25∶75）,flow rate was 1.0mL·min^-1;The detection wave-length was 262nm,column temperature was room temperature and sample size was 20μL.The theoretical plate number no less than 3000.RESULTS The RSD of within-day precision and day to day precision of mice plasma and tissue were all less than 4%,and the method recovery of plasma was more than 97% and the RSD was less than 3%.The method recovery of tissues were all more than 95% and the RSD less than 4%,and these can satisfied the requirement of biological specimen analytical method.CONCLUSION The HPLC method was convenient and accurate,endogenous material had no interference.It was suitable for determination of itraconazole in a large number of biological specimen.