| 基于COI 条形码的中药材蛇蜕及其易混伪品的DNA 分子鉴定 |
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| 引用本文： | 石林春,陈俊,刘冬,张红印,贾静,张辉,姚辉.基于COI 条形码的中药材蛇蜕及其易混伪品的DNA 分子鉴定[J].世界科学技术-中医药现代化,2014,16(2):284-287. |
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| 作者姓名： | 石林春 陈俊 刘冬 张红印 贾静 张辉 姚辉 |
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| 作者单位： | 中国医学科学院北京协和医学院/药用植物研究所 北京 100193;中国医学科学院北京协和医学院/药用植物研究所 北京 100193;长春中医药大学中医药与生物工程研发中心 长春 130117;长春中医药大学中医药与生物工程研发中心 长春 130117;长春中医药大学中医药与生物工程研发中心 长春 130117;长春中医药大学中医药与生物工程研发中心 长春 130117;中国医学科学院北京协和医学院/药用植物研究所 北京 100193 |
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| 基金项目： | 科学技术部国家高技术研究发展计划（“863 计划”）（2012AA021602）：基于DNA 条形码的珍稀药用、野生等资源快速检测技术及产品开发，负责人：陈士林。 |
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| 摘 要： | 目的：对中药材蛇蜕及其易混伪品进行分子鉴定，以确保该中药材的临床用药安全。方法：以COI 序列作为DNA 条形码，对蛇蜕原药材进行DNA 提取，PCR 扩增和序列测定，对13 个物种的68 份样品进行DNA Barcoding Gap 分析和系统聚类分析。结果：蛇蜕3 种基原黑眉锦蛇Elaphe taeniura Cope、锦蛇Elaphe carinata（Guenther）和乌梢蛇Zaocys dhumnades（Cantor）具有DNA Barcoding Gap，在邻接（NJ）系统聚类树上分别聚为独立一枝。结论：COI 作为DNA 条形码，不仅可以鉴定中药材蛇蜕的3 种基原，而且可以区分蛇蜕及其易混伪品，表明DNA 条形码可以用于中药材蛇蜕的鉴定。
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| 关 键 词： | 蛇蜕 DNA 条形码 COI 鉴定 |
| 收稿时间： | 2/7/2014 12:00:00 AM |
| 修稿时间： | 2014/2/18 0:00:00 |
Molecular Identification of Serpentis Periostracum and Its Adulterants Based on COI sequence |
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| Shi Linchun,Chen Jun,Liu Dong,Zhang Hongyin,Jia Jing,Zhang Hui and Yao Hui.Molecular Identification of Serpentis Periostracum and Its Adulterants Based on COI sequence[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2014,16(2):284-287. |
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| Authors: | Shi Linchun Chen Jun Liu Dong Zhang Hongyin Jia Jing Zhang Hui and Yao Hui |
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| Institution: | Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing 100193,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing 100193,China;Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China;Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China;Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China;Development Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Chinese Medicine, Changchun 130117, China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing 100193,China |
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| Abstract: | Objective: This study aimed to distinguish Serpentis Periostracum from its adulterants, which will provide the basis for its safe application. Methods: Here, COI sequences of 68 samples from 13 species were PCR amplified and sequenced. Furthermore, the DNA Barcoding Gap and phylogenetic cluster analysis were carried out. Results:The results exhibited that the COI sequences of all the three origin animals of Serpentis Periostracum have DNA Barcoding Gap. For phylogenetic cluster analysis, all the three origin animals showed monophyletic and every species can be discriminated clearly. Conclusion: COI is an effective DNA barcode for the identification of Serpentis Periostracum. |
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| Keywords: | Serpentis Periostracum COI DNA barcoding identification |
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