| 苍耳子药材及其混伪品ITS2 序列鉴定研究 |
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| 引用本文： | 王俊,刘霞,张雅琴,宋明,林韵涵,马孝煕,孙伟,向丽,胡志刚,邬兰,张雪琼,胡伟毅.苍耳子药材及其混伪品ITS2 序列鉴定研究[J].世界科学技术-中医药现代化,2014,16(2):329-334. |
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| 作者姓名： | 王俊 刘霞 张雅琴 宋明 林韵涵 马孝煕 孙伟 向丽 胡志刚 邬兰 张雪琼 胡伟毅 |
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| 作者单位： | 武汉理工大学化学工程学院 武汉 430070；中国中医科学院中药研究所 北京 100700;武汉理工大学化学工程学院 武汉 430070；中国中医科学院中药研究所 北京 100700;武汉理工大学化学工程学院 武汉 430070；中国中医科学院中药研究所 北京 100700;武汉理工大学化学工程学院 武汉 430070；中国中医科学院中药研究所 北京 100700;武汉理工大学化学工程学院 武汉 430070；中国中医科学院中药研究所 北京 100700;武汉理工大学化学工程学院 武汉 430070；中国中医科学院中药研究所 北京 100700;中国中医科学院中药研究所 北京 100700;中国中医科学院中药研究所 北京 100700;中国中医科学院中药研究所 北京 100700;中国中医科学院中药研究所 北京 100700;武汉理工大学化学工程学院 武汉 430070;连云港出入境检验检疫局江苏 连云港 222042 |
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| 基金项目： | 财政部教育部中央高校基本科研业务费专项青年教师探索项目（2012-IV-107）：湖北麦冬对ob/ob 小鼠2 型糖尿病的治疗作用及其作用机理研究，负责人：刘霞。 |
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| 摘 要： | 目的：应用ITS2 序列快速并准确地鉴定中药材苍耳子，为其药材质量、用药安全提供保障。方法：提取苍耳子药材及其混伪品的基因组DNA、扩增ITS2 序列并测序，采用软件CodonCode Aligner V 4. 2 对测序峰图进行校对拼接，并对峰图进行质量控制。应用MEGA 5.0 软件计算种内种间Kimura 2-parameter（K2P）遗传距离,构建邻接（NJ）系统聚类树。结果：苍耳子药材基原物种种内K2P 遗传距离为0，药材基原物种与其他混伪品的K2P 遗传距离分布于0.009~0.542；NJ 树结果显示苍耳子药材与其混伪品均可明显区分。结论：ITS2 序列适用于中药材苍耳子及其混伪品鉴别，进一步验证了DNA 条形码技术鉴定中药材的有效性。
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| 关 键 词： | 苍耳子 DNA 条形码 ITS2 鉴定 |
| 收稿时间： | 2/7/2014 12:00:00 AM |
| 修稿时间： | 2014/2/18 0:00:00 |
Identification of Xanthii Fructus and Its Adulterants Based on ITS2 Sequence |
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| Wang Jun,Liu Xi,Zhang Yaqin,Song Ming,Lin Yunhan,Ma Xiaoxi,Sun Wei,Xiang Li,Hu Zhigang,Wu Lan,Zhang Xueqiong and Hu Weiyi.Identification of Xanthii Fructus and Its Adulterants Based on ITS2 Sequence[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2014,16(2):329-334. |
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| Authors: | Wang Jun Liu Xi Zhang Yaqin Song Ming Lin Yunhan Ma Xiaoxi Sun Wei Xiang Li Hu Zhigang Wu Lan Zhang Xueqiong and Hu Weiyi |
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| Institution: | School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;;School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;;School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;;School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;;School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;;School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Chemical Engineering, Wuhan University of Technology, Wuhan 430070, China;Lianyungang Entry-exit Inspection and Quarantine Bureau, Lianyungang, Jiangsu 222042, China |
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| Abstract: | Objective: To identify Xanthii Fructus and secure its quality and safety in medication. Methods: Total genomic DNA was extracted from Xanthii Fructus and its adulterants. ITS2 sequences were amplified, and purified PCR products were sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner V 4.2. The Kimura 2-Parameter (K2P) distances were calculated using MEGA 5.0. The neighbor-joining (NJ) phylogenetic trees were constructed. Results: The intraspecific genetic distances of Xanthii Fructus were 0. The interspecific genetic distances between Xanthii Fructus and its adulterants were ranged from 0.009 to 0.542. The NJ tree showed that Xanthii Fructus could differ from its adulterants obviously. Conclusion: ITS2 can be used to identify Xanthii Fructus from its adulterants effectively, and our study further confirmed the effectiveness of ITS2 to identify traditional Chinese medicinal materials. |
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| Keywords: | Xanthii Fructus DNA barcoding ITS2 identification |
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