瘦素协同纤连蛋白对早孕绒毛细胞滋养细胞浸润特性的影响

目的:探讨瘦素(leptin)、纤连蛋白(FN)对细胞滋养细胞浸润能力的影响及其协同作用。方法:①取孕6-8周的人工流产妊娠绒毛,分离、培养细胞滋养细胞并经光镜、电镜、免疫细胞化学鉴定。②用RT-PCR方法检测细胞滋养细胞中瘦素的表达。③应用体外培养侵袭模型检测瘦素、FN及二者协同时对细胞滋养细胞浸润能力的影响。结果:①分离、纯化所得细胞滋养细胞有瘦素mRNA表达。②瘦素组、FN组和瘦素与FN协同组浸润穿过matrigel的滋养细胞数均显著多于对照组(P<0.01),其中瘦素与FN协同组滋养细胞浸润能力最强。结论:①瘦素能增强细胞滋养细胞的侵袭能力。②FN对细胞滋养细胞的浸润具有趋化作用。③瘦素与FN对细胞滋养细胞的浸润有正协同作用。

Effect of Leptin-Fibronectin Synergy on Trophoblast Invasion

Objective: To study the effects of leptin, fibronectin and their synergy on cytotrophoblast invasion activity. Methods: Cytotrophoblasts (CTB) were isolated from chorionic villi at 6-8 weeks pregnancy, evaluated by light, electron microscope and immunocytochemistry and cultured in vitro. Using transwell invasion model coated with matrigel, the change of cytotrophoblast invasion ability was observed before/after adding leptin, FN or leptin and FN into culture medium. Results: The isolated cells cytotrophoblasts and they expressed leptin mRNA. The number of penetrated-membrane-cells in leptin, FN, leptin FN groups were significantly higher than control group. The number of invasive CTB in leptin FN group was larger than that in leptin group or FN group. Conclusions: Leptin can increase the invasion ability of cytotrophoblast. FN can promote the invasion ability of trophoblasts. Leptin-FN synergy can significantly enhance the CTB invasion ability than FN or leptin alone. It proves that CTB invasion procedure is modulated by multiple factors of cell-to-cell and cell-to-matrix interaction.