| HPLC法测定金玄痔科熏洗散有效物质部位中金丝桃苷的含量 |
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| 引用本文： | 章文颖,周璐,王辉,李亚平,刘焱文.HPLC法测定金玄痔科熏洗散有效物质部位中金丝桃苷的含量[J].中国药房,2011(7):628-629. |
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| 作者姓名： | 章文颖 周璐 王辉 李亚平 刘焱文 |
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| 作者单位： | 湖北中医药大学中药资源与中药化学省级重点实验室;马应龙药业集团股份有限公司博士后工作站; |
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| 基金项目： | 马应龙药业集团肛肠基金项目 |
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| 摘 要： | 目的:建立测定金玄痔科熏洗散有效物质部位中金丝桃苷含量的方法,为完善该中药复方制剂的质量标准提供参考依据。方法:采用高效液相色谱法。色谱柱为Venusil XBPC18(250mm×4.6mm,5μm),流动相为乙腈-0.2%磷酸溶液(18∶82),检测波长为360nm,柱温为25℃,流速为1.0mL·min-1。结果:金丝桃苷进样量在0.0436～0.4360μg范围内与峰面积积分值呈良好的线性关系(r=0.9995);平均回收率为98.36%,RSD=0.79%(n=6)。结论:本方法操作简便、可靠性强,可为完善金玄痔科熏洗散的质量标准提供参考依据。
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| 关 键 词： | 金玄痔科熏洗散 高效液相色谱法 金丝桃苷 含量测定 |
Content Determination of Hyperin in Effective Part of Jinxuan Zhike Xunxi San by HPLC |
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| ZHANG Wen-ying,ZHOU Lu,WANG Hui,LI Ya-ping,LIU Yan-wen ZHOU Lu.Content Determination of Hyperin in Effective Part of Jinxuan Zhike Xunxi San by HPLC[J].China Pharmacy,2011(7):628-629. |
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| Authors: | ZHANG Wen-ying ZHOU Lu WANG Hui LI Ya-ping LIU Yan-wen ZHOU Lu |
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| Institution: | ZHANG Wen-ying,ZHOU Lu,WANG Hui,LI Ya-ping,LIU Yan-wen(Key Laboratory of Resource Science and Chemistry of Traditional Chinese Medicine,Hubei University of Traditional Chinese Medicine,Wuhan 430061,China) ZHOU Lu(Postdoctoral Research Center,Mayinglong Pharmaceutical Group Co.,Ltd.,Wuhan 430064,China) |
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| Abstract: | OBJECTIVE： To establish a determination method of hyperin in effective part of Jinxuan zhike xunxi san, and to provide reference standards for improving the quality standard of Chinese herbal compound preparation. METHODS： The content of hyperin was determined by HPLC. Venusil XBP C18（250 mm×4.6 mm, 5μm）column was used and the detection wavelength was set at 360 nm. The mobile phase was consisted of acetonitrite-0.2% phosphatic acid （18：82） with flow rate of 1.0 mL·min-1 and column temperature at 25℃ RESULTS： The linear range of hyperin was 0.043 6-0.436 0μg（r=0.999 5） with an average recovery of 98.36 % （RSD = 0.79 %, n =6）. CONCLUSION： The method is simple, reliable and can provide reference standard for improving the quality standards of Jinxuan zhike xunxi san. |
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| Keywords: | Jinxuan zhike xunxi san HPLC Hyperin Content determination |
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