滋肾降糖丸对小鼠骨髓基质干细胞成脂分化的影响

目的:研究滋肾降糖丸对小鼠骨髓基质干细胞(MSC)模型成脂分化的影响。方法:小鼠灌胃滋肾降糖丸(1.5g·kg-1),每天1次,连续7d,最后一天在灌胃2h后收集含药血清。随后用油红O染色法检测上述含药血清对MSC成脂诱导分化的影响。最后用实时荧光定量聚合酶链式反应(Q-RT-PCR)方法进一步测定含药血清对MSC成脂诱导过程中相关关键基因过氧化物酶体增殖物激活γ2受体(PPARγ2)与人脂肪酸结合蛋白4(FABP4)表达的影响。结果:含药血清可显著抑制MSC的成脂分化,显著抑制MSC成脂诱导分化第3、6天后关键基因PPARγ与FABP4的表达。结论:滋肾降糖丸具有抑制MSC成脂分化的作用,其作用机制可能与抑制MSC上PPARγ2与FABP4的表达有关。

Effect of Zishen Jiangtang Pills on Adipogenic Differentiation of Mice MSC and Its Mechanisms

OBJECTIVE： To investigate the effect of Zishen jiangtang pills on adipogenic differentiation of mice mesenchymal stem cells （MSC） and its mechanisms. METHODS： Mice were given Zishen jiangtang pill via i.g. gtt at a dose of 1.5 g.kg-1 once a day for a continuous week. Serum sample was collected 2 h after last administration. Then, effects of serum containing active com- ponents on adipogenic differentiation of MSC were investigated by means of oil red O staining. The expressions of PPAR72 and FABP4 were determined by real-time Q-RT-PCR method during adipogenic differentiation of MSC. RESULTS： The serum contain- ing active components inhibited the adipogenic differentiation of MSC significantly. The expressions of PPAR）,2 and FABP4 were significantly decreased after 3 and 6 days of adipogenesis induction. CONCLUSION： Zishen jiangtang pills can inhibit adipogenic differentiation of MSC, which might related to the expressions inhibition of PPARy2 and FABP4.