肺腺癌细胞Tiam1，E-cadherin和Vimentin的表达及与上皮间质转化的关系

目的：探讨T淋巴瘤侵袭转移诱导因子1（Tiaml）及E—cadherin，Vimentin蛋白与上皮间质转化（epithelialandmesenchymaltransition，EMT）在肺腺癌细胞中的表达关系的临床意义。方法：从112例恶性胸水中提取肺腺癌细胞为研究对象，以39例良性胸水中增生上皮细胞为对照，常规液基细胞薄层（TCT）技术、H＆E染色、免疫细胞化学染色检测胸水中的肺腺癌细胞。采用免疫细胞化学方法检测细胞中Tiaml、E钙粘素蛋白、Vimentin的表达。结果：112例恶性胸水标本中，75例Tiaml在肺腺癌细胞中表达阳性，阳性率为67．0％。E-cadherin及Vimentin蛋白在肺腺癌细胞中的表达率分别为81／112（72．3％）和94／112（83．9％）。结果显示，Tiaml在肺腺癌细胞中的表达的增强伴随E—cadhenn表达的下降及Vimentin的表达增强。Tiaml和E-cadhefin在增生上皮细胞中阴性表达。结论：Tiaml在肺腺癌细胞中阳性表达，其影响肿瘤发展的机制可能与上皮间质转化发生有关。

Relationship between Tiaml, E-cadherin, Vimentin expression and EMT in lung adenocarcinoma cells

Objective：To investigate the clinical significance of relationship between Tiaml （Tqymphoma invasion and metastasis inducing factor 1 ）, E-cadherin, Vimentin and EMT （epithelial and mesenchymal transition） in lung adenocarcinoma ceils. Methods： Lung adenocarcinoma cells were obtained to analyze in 112 cases of malignant pleural effusion. 39 hyperplasia epithelium cells of non-malignant pleural effusions were obtained as control. Malignant pleural effusions were analyzed by thin-prep cytology test （TCT） , H＆E staining and immunocytochemistry staining. Immunocytochemistry staining was used to test Tiaml, E-cadherin, and Vimentin. Results： Tiaml positive expression was found in 75 of 112 （67. 0% ） cases malignant pleural effusions. E-cadherin and vimentin expression was found in 81/112 （72.3%） and 94/112 （83.9%） cases, respectively. Our data showed elevated levels of Tiaml were accompanied by down regulation of E-cadherin and the up regulation expression of vimentin in lung adenocarcinoma cells. Hyperplasia epithelium cells from nonmalignant pleural fluid were uniformly negative for E-cadherin and Tiaml. Conclusion：Our study shows Tiaml positive expression in lung adenocarcinoma ceils may be related to EMT.