开郁胶囊对抑郁动物模型的影响

目的:开郁胶囊是本室自行研制的由8种传统中药组成的中药新复方制剂,本研究观察开郁胶囊对数种抑郁动物模型的影响。方法:采用ICR小鼠随机分为对照组、开郁胶囊低、中、高剂量组(按生药量计为6.6,13.2,26.4 g.kg-1)及阳性对照药帕罗西汀组。连续ig 14 d,于末次给药1.5 h后采用小鼠悬尾、强迫游泳、5-羟色胺酸(5-HTP)诱导甩头及利血平拮抗实验动物模型,评价开郁胶囊的抗抑郁作用。结果:与对照组相比,开郁胶囊中、高剂量组能显著缩短小鼠悬尾不动时间(P<0.05);开郁胶囊高剂量组显著增加5-HTP诱导的小鼠甩头次数(P<0.01),缩短甩头潜伏期(P<0.05)。注射利血平后动物出现利血平化,即眼睑下垂、体温下降及活动抑制;开郁胶囊中、高剂量ig 14 d,可改善利血平化小鼠的眼睑下垂及活动抑制(P<0.01),对体温下降亦有显著的拮抗作用(P<0.01)。结论:开郁胶囊对拟抑郁症小鼠模型具有明显的拮抗作用,其作用机制可能与增强5-羟色胺能和去甲肾上腺素能神经功能有关。

Effects of Kaiyu Capsule on Depression-like Animal Models

Objective: Kaiyu capsule is a new compound preparation which consists of 8 traditional Chinese herbs. The purpose of the study was to investigate the effects of Kaiyu capsule on depression-like animal models. Method : ICR mice were randomly allocated to five groups: control, Kaiyu capsule low, middle and high dose groups (6.6,13.2, 26.4 g ·kg^-1 accordingly), and Paroxetine (positive control). After intragastric administration for 14 d, the antidepressant effect of Kaiyu capsule was evaluated by using mice tail suspension test, mice forced swimming test, 5-hydroxytryptophan (5-HTP)-induced head-twitches and reserpine reversal model in mice. Result : Compared with control group, the middle and high doses of Kaiyu capsule significantly decreased the accumulative immobility time in mice tail suspension test(P<0.05). High dose Kaiyu capsule obviously increased the head-twitching number induced by 5-HTP(P<0.01), and shortened the latency period(P<0.05). After sc reserpine, the mice appeared physical signs such as blepharoptosis, akinesia and hypothermia. The middle and high doses of Kaiyu capsule significantly ameliorated the blepharoptosis and akinesia(P<0.01), and decreased the range of hypothermia in reserpine-induced model mice(P<0.01). Conclusion : Kaiyu capsule has the significant antidepressant effect, and the mechanisms may be related to the enhancement of serotonergic and noradrenergic functions.