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| 斑蝥素对HL—60细胞和QGY7703细胞的作用研究 | |
| 引用本文： | 金伟,王亚威,周宗祥,应康,谢毅,毛裕民.斑蝥素对HL—60细胞和QGY7703细胞的作用研究[J].中医药学报,2001,29(4):42-43. |
| 作者姓名： | 金伟 王亚威 周宗祥 应康 谢毅 毛裕民 |
| 作者单位： | 1. 复旦大学遗传工程国家重点实验室,复旦大学生命科学学院,上海,200433 2. 黑龙江省中西医结合研究所,黑龙江,哈尔滨,150036 3. 联合基因科技集团有限公司,上海,200092 |
| 摘 要： | 采用MTT法和流氏细胞仪技术，研究了斑蝥素对HL－60细胞和QGY7703细胞的存活率和细胞周期分布影响。结果表明，斑蝥素对HL－60细胞的IC50是15．34μmol/L，对QGY7703细胞的IC50是18．54μmol/L。按蝥素处理HL－60细胞和QGY7703细胞24h后，G2－M期细胞分布都有所增加，G0－G1期细胞分布都有所降低；斑蝥素处理HL－60细胞24h后，G0－G1期峰前有亚二倍体峰出现，说明斑蝥素在所用剂量下降够诱导HL－60细胞发生凋亡。 |
| 关 键 词： | 斑蝥素 细胞存活率 细胞周期 HL－60细胞 QGY7703细胞 |
The effect of cantharidism on HL-60 cell and QGY7703 cell |
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| Jin wei,et al State Key Laboratory of Genetic Engineering,Fudan University,Shanghai.The effect of cantharidism on HL-60 cell and QGY7703 cell[J].Acta Chinese Medicine and Pharmacology,2001,29(4):42-43. | |
| Authors: | Jin wei State Key Laboratory of Genetic Engineering Fudan University Shanghai |
| Institution: | Jin wei,et al State Key Laboratory of Genetic Engineering,Fudan University,Shanghai 200433 |
| Abstract: | The cell livability and cell cycle of HL-60 cells and QGY7703 cells treated by cantharidin was observed by MTT colorimetry and flow cytometry. We found that the IC 50 of HL-60 cells to cantharidin is 15.34 umol.L -1 and the IC 50 of QGY7703 cells to cantharidin is 18.54 umol.L -1 . After treated by cantharidin for 24 hours, G 2 -M period of HL-60 cells and QGY7703 cells all increased, G 0 -G 1 period of HL-60 cells and QGY7703 cells all decreased; The appearance of subdiploid peak proved the apoptosis inducing activity of cantharidin when it treated HL-60 cells for 24 hours. |
| Keywords: | cantharidin cell livability cell cycle |
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