针刺对部分背根切断后大鼠脊髓背角生长相关蛋白GAP43表达的影响

本研究用单侧腰骶背根腰L2～骶S2切断保留L5的大鼠模型观察针刺对脊髓背角内生长相关蛋白GAP43表达的影响。结果观察到切断一侧L2，3，4，6及S1，2背根后，L5水平脊髓背角Ⅱ板展与对侧及对照组相比生长相关蛋白GAP3免疫阳性着色加深，而原位杂交结果显示背角阳性GAP43mRNA阳性加强。进行针刺治疗后可促进受损侧L5脊髓背角Ⅱ板层GAP3免疫标记进一步加强，L5脊髓背角GAP43杂交阳性信号强度屯进一步增强。这些结果提示：①一定条件下的神经系统损伤可以诱发中枢神经系统由GAP43介导的可塑性变化，②针刺可促进这种GAP3介导的可塑性变化。

Acupuncture Effect on GAP43 Expression in Spinal Cord Dorsal Area of Spinal Cord Partial Deafferental Rat Model

Experiments were performed on rat model given unilateral lumbosacral dorsal rhizotomical opera tion from L2 to S2 with sparing L5. The effect of acupuncture on expression of GAP3 in dorsal area of spinal cord was observed with histocytochemistrical and in situ hybridizational methods. The results showed that after unilateral dorsal rhizotomied, the expression of GAP43 in lamina Ⅱ of spinal cord (L5) was significantly more than contralateral or control group. When acupuncture trement was giv en, the expression of GAP43 was elevated to higher level. These results implied that ① in some condi tion nerve system injury can induce the increasing GAP43 expression of neurons which mediate the plastisity of central nerve system, ② the acupuncture treatment can regulate the plasticity of the nerve system through GAP43 mechanism