镇痛效应对电灸调节荷瘤大鼠NK细胞活性的影响

为探讨针刺镇痛效应与针灸调节免疫作用间的相关性，以大鼠NK细胞活性变化为指标，通过荷瘤抑制大鼠免疫功能，观察电灸、电灸结合D-苯丙氨酸等对荷瘤大鼠和电针镇痛不同敏感状态荷瘤大鼠NK细胞活性的影响。结果显示：W256瘤株荷瘤大鼠NK细胞活性明显低于正常大鼠，电灸能明显改善其NK细胞活性。2Hz电针刺激大鼠“足三里”穴时，存在镇痛有效群（痛阈升高大鼠）和无效群（痛阈无变化大鼠）之别，电灸“关元”穴，能明显提高电针镇痛有效群荷瘤大鼠的NK细胞活性，但不能改善镇痛无效群荷痛大鼠的NK细胞活性，经加用吗啡分解酶抑制剂D-苯丙氨酸（250mg／kg，ip）后，镇痛有效群荷瘤大鼠的NK细胞活性有更大程度的提高，接近正常大鼠的水平。镇痛无效群荷瘤大鼠的NK细胞治性也有明显改善。单用同等剂量的D-苯丙氨酸并不影响NK细胞活性。本工作结果表明，电灸改善荷瘤大鼠NK细胞活性与大鼠本身对电针镇痛的敏感性有关，针刺镇痛效应可能影响其对免疫功能的调制作用。

Influence of Acupuncture Analgesia on NK Cytotoxicity of Tumor-Beared Rats Induced by Electro-Moxibustion

To investigate the correlation between acupuncture analgesia and immunomodulation by acupunc ture, the splenic natural killer cell cytotoxicity in normal and tumor beared rats was detected, results of which were compared among electro-moxibustion treatment(EM), EM plus D-phenylalanine and D phenylalanine treatment in acupuncture analgesia responding rats and non-responding rats. The results showed that the NK cytotoxicity was much lower in W256 tumorbeared rats than that in normal group, which was significantly upgraded by electrcrmoxibustion treatment. There existed individual diflerences in analgesic effect in rats(acupuncture analgesia responders and non-responders) by the 2 Hz electro-stimulation at Zusanli(ST 36). Electro-moxibustion at Guanyuan (CV 4) greatly improved the tumorbeared rat's NK cell activity in AA responders, but failed to regulate that in AA non-re sponding rats. The NK cytotoxicity was remarkab1ely improved near to normal level in AA responders and significantly upgraded in AA nonresponders when treated by EM and 250mg/kg D-phenylalanine intraperitoneal injection. 250 mg/kg D-phenylalanine itself did not modulate the NK cell activity. The work indicates that modulation of NK cell activity by moxibustion is related to the rat's individual dif ferences in acupuncture analgesic effect. Acupuncture analgesia does affect the regulation of NK cyto toxicity by moxibustion