Simultaneous determination of T,B, and mixed rosette-forming lymphocytes in human peripheral blood

Preliminary treatment of erythrocytes in suspension with trypan blue did not affect their ability to take part in the spontaneous rosette formation test, but enabled them to be distinguished when mixed with other erythrocytes after staining with methyl green-pryronine in fixed preparations. The presence of a small subpopulation of lymphocytes binding both types of indicator particles was demonstrated by the use of a mixture of these, erythrocytes with erythrocytes sensitized by antibodies and complement.Department of Immunology and Laboratory of Immunomorphology, Research Center, N. I. Pirogov Second Moscow Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR Yu. M. Lopukhin.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 6, pp. 714–716, June, 1977.