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| Anti-miR-155反义寡核苷酸对人肺鳞癌H157细胞增殖的影响 | |
| 引用本文： | 曹学武,安江洪,陈正堂. Anti-miR-155反义寡核苷酸对人肺鳞癌H157细胞增殖的影响[J]. 西南国防医药, 2008, 18(3): 322-324 |
| 作者姓名： | 曹学武 安江洪 陈正堂 |
| 作者单位： | 第三军医大学新桥医院全军肿瘤研究所,重庆,400042 |
| 基金项目： | 国家自然科学基金项目(30672076)、国家重点基础研究发展"863"计划资助(2007AA02Z129) |
| 摘 要： | 目的：观察anti-miR-155反义寡核苷酸（AMOs）对肺鳞癌H157细胞增殖的影响。方法：将H157细胞分为对照组和AMOs处理组，AMOs处理组采用AMOs抑制H157细胞内miR-155的活性，液闪计数仪测定[^3H]-TdR掺入量，MTT法测定细胞增殖抑制率，流式细胞仪测定细胞周期。结果：与对照组相比，AMOs显著减少H157细胞[^3H]-TdR掺入量（P〈0．01），随着浓度从10nmol／L逐渐增加至于100nmol／L，H157细胞[^3H]-TdR掺入量亦随之减少（P〈0．01）；AMOs显著抑制H157细胞的增殖（P〈0.01），使G0／G1细胞比例显著增加，G2／M期细胞比例显著减少（P〈0．01）。结论：AMOs通过抑制H157细胞内高水平表达miR-155的活性，显著抑制H157细胞的增殖。 |
| 关 键 词： | H157 miR-155 反义寡核苷酸 细胞增殖 |
| 文章编号： | 1004-0188(2008)03-0322-03 |
| 修稿时间： | 2008-02-26 |
Effect of anti-miR-155 oligonucleotides on proliferation of human lung squamous cell carcinoma cell line H157 |
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| CAO Xue-wu,AN Jiang-hong,CHEN Zheng-tang. Effect of anti-miR-155 oligonucleotides on proliferation of human lung squamous cell carcinoma cell line H157[J]. Medical Journal of National Defending forces in Southwest China, 2008, 18(3): 322-324 | |
| Authors: | CAO Xue-wu AN Jiang-hong CHEN Zheng-tang |
| Affiliation: | CAO Xue-wu,AN Jiang-hong,CHEN Zheng-tang Cancer Institute of PLA,Xinqiao Hospital,Third Military Medical University,Chongqing,400042,China. |
| Abstract: | Objective:To determine the effect of anti-miR-155 oligonucleotides(AMOs) on proliferation of human lung squamous cell carcinoma cell line H157.Methods:Cultured H157 cells were divided into two groups: control group and AMOs group.In AMOs group,AMOs were used to inhibit the activity of miR-155.-TdR incorporation was detected by liquid scintillation counting,MTT assay was used to examine the inhibition percentage of AMOs to the proliferation of H157 cells and cell cycle was measured by flow cytometry.Results:... |
| Keywords: | H157 miR-155 |
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