| 人关节软骨脱细胞基质的制备 |
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| 引用本文： | 张建党,卢世璧,黄靖香,袁枚,赵斌,孙明学,崔雪梅. 人关节软骨脱细胞基质的制备[J]. 中国矫形外科杂志, 2005, 13(4): 276-277 |
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| 作者姓名： | 张建党 卢世璧 黄靖香 袁枚 赵斌 孙明学 崔雪梅 |
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| 作者单位： | 解放军总医院骨科研究所,北京,100853 |
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| 基金项目： | 国家863资助项目(编号:2002AA205021) |
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| 摘 要： | 目的制备人关节软骨脱细胞基质。方法切取人关节软骨,对之冷冻干燥后,采取化学去污剂TritonX100及DNA酶和RNA酶等试剂制备脱细胞的人关节软骨。做HE、番红0及软骨蛋白聚糖(aggrecan)免疫组化染色等方法进行检测。结果HE染色、番红0染色均显示细胞陷窝内己无细胞结构番红花0染色阳性;软骨蛋白聚糖免疫组化染色阳性。结论人关节软骨冻干后,经去污剂酶等处理方法可脱去软骨的细胞成分,并且保留了软骨细胞外基质,成功制备了人关节软骨脱细胞基质。
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| 关 键 词： | 脱细胞基质 关节软骨 去污剂 冷冻干燥 |
| 文章编号： | 1005-8478(2005)04-0276-02 |
| 修稿时间： | 2004-08-30 |
Development of human articular cartilage acellular matrix |
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| ZHANG Jian-dang,LU Shi-bi,HUANG Jing-xiang,et al.. Development of human articular cartilage acellular matrix[J]. The Orthopedic Journal of China, 2005, 13(4): 276-277 |
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| Authors: | ZHANG Jian-dang LU Shi-bi HUANG Jing-xiang et al. |
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| Affiliation: | ZHANG Jian-dang,LU Shi-bi,HUANG Jing-xiang,et al.Institute of Orthopedics,General Hospital of PLA,Beijing 100853 |
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| Abstract: | Objective:To develop a cell extraction process to prepare human articular cartilage acellular matrix.Method:Fresh human articular cartilage was subjected to modified four-step detergent and enzymatic extraction process after lyophilization,then,the specimens were assessed by staining with haematoxylin-eosin,safranin-O (for GAGs),and by the immunolocation of aggrecan.Result:Light microscopy confirmed that the celluar constituents of the specimens were removed,the specimens stained positively for GAGs and for aggrecan.Conclusion:The modified four-step detergent and enzymatic extraction process with lyophilization can remove the cellular constituents from human articular cartilage,in addition,structional extracellular matrix can be retained. |
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| Keywords: | Acelluar matrix Articular cartilage Detergent Lyophilization |
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