慢性乙型肝炎患者血清磷脂酰肌醇-4-磷酸酶的表达与HBV DNA载量的相关性

目的探讨慢性乙型肝炎患者血清磷脂酰肌醇-4-磷酸酶(PI4KA)表达与HBV载量的关系。方法收集2012年6月-2013年4月在青岛市立医院就诊的180例慢性乙型肝炎患者的血清,利用荧光定量PCR检测其HBV DNA的载量,根据其HBV DNA载量的高低,分为3组,每组60例:高病毒载量组(HBV DNA1×107拷贝/ml)、中病毒载量组(1×105拷贝/ml≤HBV DNA≤1×107拷贝/ml)及低病毒载量组(HBV DNA1×105拷贝/ml),同时以60名健康人作对照。采用ELISA法,检测各组外周血清中的PI4KA的浓度。多组间血清HBV DNA定量的比较采用单因素方差分析,进一步两两比较采用LSD-t检验。PI4KA水平与HBV DNA载量之间的相关性分析采用Spearman检验。结果健康人血清PI4KA的浓度为(2.29±0.75)ng/ml;低、中、高病毒载量组血清PI4KA的浓度分别为(2.73±0.71)、(3.52±0.78)及(4.72±0.77)ng/ml。慢性乙型肝炎患者不同病毒载量组与健康对照组间血清PI4KA的浓度差异有统计学意义(F=119.958,P0.01),并且不同病毒载量组都显著高于对照组。随着HBV DNA载量的升高,PI4KA的浓度增加,两者呈正相关(r=0.758,P0.01)。结论慢性乙型肝炎患者血清PI4KA的浓度与HBV DNA载量密切相关,这一结果提示PI4KA可能在HBV DNA的复制中起到重要作用。

Correlation between expression of PI4KA in serum and HVB DNA load in patients with chronic hepatitis B

ObjectiveTo investigate the relationship between the expression of phosphatidylinositol 4-kinase III alpha （PI4KA） in serum and HBV DNA load in patients with chronic hepatitis B （CHB）. MethodsSerum samples were collected from 180 patients with CHB, whose HBV DNA loads were determined by fluorescence quantitative PCR. According to the serum HBV DNA loads, the 180 patients were divided into three groups： high viral load group （HBV DNA load 〉107 copies/ml）, medium viral load group （105 copies/ml≤HBV DNA load≤107 copies/ml）, and low viral load group （HBV DNA load 〈105 copies/ml）, while the serum samples of 60 healthy people were selected as control group. Enzyme-linked immunosorbent assay was used to determine the concentration of PI4KA in peripheral serum. ResultsThe concentrations of serum PI4KA were（2.29±0.75）ng/ml in the control group, （2.73±0.71）ng/ml in the low viral load group, （3.52±0.78）ng/ml in the medium viral load group, and （4.72±0.77）ng/ml in the high viral load group. There were significant differences in serum PI4KA concentration between the patient groups with different viral loads and control group （F=119.958, P〈0.01）, and the PI4KA levels in the three viral load groups were all significantly higher than those in the control group. The HBV DNA load and PI4KA concentration presented a positive correlation with each other （r=0.758, P〈0.01） - serum PI4KA level would be elevated with the increase in HBV DNA load. ConclusionThe concentration of serum PI4KA is closely correlated with the HBV DNA load in patients with CHB, which suggests that PI4KA may play a crucial role in the HBV DNA replication.