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| 牛皮蝇不同发育阶段五种同工酶的比较分析 | |
| 引用本文： | 张立华,刘德山. 牛皮蝇不同发育阶段五种同工酶的比较分析[J]. 兰州大学学报(医学版), 1987, 0(3) |
| 作者姓名： | 张立华 刘德山 |
| 作者单位： | 兰州医学院寄生虫学教研室，兰州医学院寄生虫学教研室 |
| 摘 要： | 本文应用聚丙烯酰胺凝胶电泳技术(PAGE):1,对我国西北地区的牛皮蝇(Hypoderma bovis)的Ⅱ、Ⅲ龄幼虫和蛹期作了酯酶(EST)、乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、葡萄糖-6-磷酸脱氢酶(G6PD)和异柠檬酸脱氢酶(ICDH)等五种同工酶的比较测定。结果表明在牛皮蝇生活史的不同发育阶段,其中四种同工酶(除ICDH外)的酶带数目和相对迁移率(R_f)均有差异;2,将专性寄生性蝇类——牛皮蝇与营自由生活的蝇类——舍蝇(Musca domestica vicina macquart)进行了相同发育阶段的五种同工酶对比,发现二者在酶带数目和R_f上均有显著差别。本文认为,在牛皮蝇不同发育阶段有着不同的控制基因以适应虫体生长代谢的需要。而寄生性蝇类与非寄生性蝇类也可由于生态环境完全不同而形成不同的代谢途径。 |
| 关 键 词： | 牛皮蝇 舍蝇 同工酶 脱氢酶 聚丙烯酰胺凝胶电泳 |
The Comparable Analysis of Five Isoenzyme-patterns in the Various Developmental Stages of Hypoderma Bovis |
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| Zhang Lihua,Liu Deshan,. The Comparable Analysis of Five Isoenzyme-patterns in the Various Developmental Stages of Hypoderma Bovis[J]. Journal of Lanzhou University (Medical Sciences), 1987, 0(3) | |
| Authors: | Zhang Lihua Liu Deshan |
| Abstract: | This paper reports results of inves- tigations dealing with the comparable analysis of isoenzyme-patterns(Lac- tate dehydrogenase, Malate dehydro- genase, Esterase, Glucose-6-phosphate, and Isocitrate dehydrogenase) in the various developmental stages of H. bo- vis by means of PAGE. The experim- ental rasults revealed that during these three Life-cycle stages of H. bovis, except ICDH, others are manifest in the change of number, density and R_f. This indicates that dif- ferent loci are operating in these three life-cycle stages. |
| Keywords: | H.bovis Musca domestica vicina isoenzymes PAGE |
| 本文献已被 CNKI 等数据库收录！ | |