国产甲基叔丁基醚亚慢性毒性实验研究

目的研究国产甲基叔丁基醚（ＭＴＢＥ）的亚慢性毒性，为职业及环境污染危害寻找有用的健康监护指标。方法采用大鼠静式吸入染毒法，４ｈ／ｄ，染毒两个月。试验设０，４００，３０００和８０００ｐｐｍ四个剂量组，每组８只ＳＤ大鼠，雌雄各半。结果尽管８０００ｐｐｍ组动物平均体重略低，但与对照组比较，差异无显著意义（Ｐ＞００５）。各染毒组动物的心、肺、肝、脾、肾脏外观及平均湿重也均未见明显影响。但８０００ｐｐｍ组的红细胞总数及血红蛋白较对照明显降低，且有剂量反应关系。在所测定的１８项血生化指标中，仅见８０００ｐｐｍ组动物的胆红素及甘油三酯明显升高。由病理结果可见，肾脏病理改变与国外报告基本一致。另见高剂量组动物中，肺及肝也有一定的病理改变。结论８０００ｐｐｍ染毒两个月，对大鼠的体重、脏器湿重无明显影响。但对血常规及某些血生化指标均有一定影响，对肺、肝、肾也有一定损伤作用。

Sub chronic Toxicological Experimental Studies on Methyl Tertiary butyl Ether(MTBE)Prepared in China

Objective To study the sub chronic toxicity of methyl tertiary butyl ether(MTBE)prepared in China and to look for a useful indicators for health surveillance.Methods MTBE was inhaled in a static status by rats four hours a day for two months,which were divided into four groups based on their exposed dose(0,400,3 000,and 8 000 ppm,respectively),each with eight rats and half for male and female.Results Average body weight was a little bit less in the rats exposed to 8 000 ppm of MTBE,but without statistical significance as compared to the controls.No significant difference in the mean weight of the heart,lung,liver,spleen,and kidney and their appearance was found.Total count of red blood cells and hemoglobin concentration significantly decreased in the group exposed to 8 000 ppm of MTBE,as compared to the controls,in a dose response manner.Only blood levels of bilirubin and triglyceride in 18 biochemical indicators increased in the group exposed to 8 000 ppm of MTBE.Pathological examinations showed that changes in kidney were basically similar to that reported abroad.In addition,certain pathological changes in the lung and liver could be seen in the rats exposed to high dose of MTBE.Conclusion After exposure to 8 000ppm of MTBE for tow months,no obvious changes in their body weight and in wet weight of their viscera were found in rats exposed to 8 000 ppm of MTBE for two months,but,certain damage to blood and some biochemical indicators could be seen.