高效液相色谱法测定五维葡钙口服溶液中维生素B_1、维生素B_2的含量

目的建立以高效液相色谱法测定五维葡钙口服溶液中维生素B1、维生素B2含量的方法。方法色谱柱为ZORBAX Eclipse XDB-C18柱(5μm,250mm×4.6mm)。流动相为0.005mol/L庚烷磺酸钠溶液(含0.5%冰醋酸和0.05%三乙胺)-甲醇(72∶28),柱温30℃,流速为1ml/min,检测波长为260nm,进样量20μl。结果维生素B1、维生素B2检测浓度的线性范围分别为7.82~23.97μg.mL-1(r=0.9996)、8.26~34.81μg.mL-1(r=0.9992);平均回收率分别为102.5%(RSD=0.6%)、99.4%(RSD=0.8%)。结论本方法简便准确、灵敏度高、结果可靠,可用于五维葡钙口服溶液中维生素B1、维生素B2的含量测定。

DETERMINATION OF VITAMIN B1, VITAMIN B2 IN WUWEIPUGAI ORAL LIQUID USING HIGH-PRESSURE LIQUID CHROMATOGRAPHY METHOD

Objective Establish the method to determine vitamin B1 and vitamin B2 in Wuweipugai oral liquid by HPLC. Methods PE Series 200 type HPLC is used, which column is ZORBAX Eclipse XDB - C18 （5 μ m, 250 mm ×4. 6 mm）, mobile phase is 0. 005 mol / L heptane solution（0. 5% acetic acid and 0.05% triethylamine） -methanol （72：28）. Wuweipugai wave is 260 nm, flow rate is 1.0 ml/min. Assay is external reference method. Results A good linear correlation was obtained when vitamin B1＇s concentration ranged from 7.82 μg/m] to 23.97 μg/mL（ r = 0. 9996 ） and vitamin B2＇s concentration ranged from 8. 26μg/mL to 34. 81μg/mL（ r = 0. 9992 ）. Recovery of B1 is 102. 5%, RSD is 0. 6% ; Recovery of B2 is 99. 4%, RSD is 0. 8%. Conclusion This method is high -sensirtive, separation effect and reproducibility are good, results are reliable, which can be applied to determine vitamin B1 and B2 in Wuweipugai oral liquid.