半乳糖诱导白内障大鼠异常晶状体蛋白X36与白内障形成的关系

目的:研究半乳糖诱导白内障大鼠异常晶状体蛋白X36的出现及其与白内障形成的关系.方法:半乳糖饲养SD大鼠,诱导白内障;选不同年龄大鼠(出生后1天、2周、8周、8个月及1.5年)作老化进程对照组;提取水溶性晶状体蛋白,双向电泳(等电聚焦/SDS-聚丙烯酰胺凝胶电泳,IEF/SDS-PAGE)得到大鼠晶状体蛋白电泳图形,考马斯亮蓝染色后扫描蛋白图形并确认各主要晶状体蛋白组分及异常晶状体蛋白X36.结果:(1)异常晶状体蛋白X36相对分子质量约27 000、等电点约4.5.(2)X36蛋白在对照组不同年龄大鼠的水溶性晶状体蛋白中均未出现.(3)X36蛋白在Ⅲ期及以后各期糖性白内障大鼠的眼晶状体水溶性蛋白中均有出现,且含量逐期增加.结论:半乳糖诱导大鼠白内障形成过程中出现异常晶状体蛋白X36,该蛋白含量随白内障程度加重而增加,与大鼠生理性老化无关.

Relationship between abnormal rat crystalline X36 and galactose-induced cataractogenesis

Objective:To study the abnormal crystalline protein X36 in cataract SD rat and to explore the relationship be-tween X36 and cataractogenesis. Methods:Cataractogenesis was induced on rat lens by galactose diet; Normal rats of differentage groups were used as controls. Water-soluble crystallins at each of the 5 Sippel stages of cataractogenesis and controlgroups were extracted and separated by two-dimensional polyacrylamide gel electrophoresis. After Comassize blue staining,thecrystallin patterns were screened and analyzed. Results: (1) The molecular weight of subunit and isoelectric point of X36 wasroughly 27 000 and 4. 5,respectively. (2) X36 did not appear in the water-soluble components of control groups. (3) X36 crys-tallin appeared in the water-soluble components at the third Sippel stage of cataractogenesis,its level increased with the ad-vancement of Sippel stage. Conclusion: (1) X36 appears in the process of cataratogenesis in glactose-induced SD rats,its levelincreases with the advancement of Sippel stage. (2) X36 cannot be detected in water-soluble crystallins in normal aging rats.