| 更昔洛韦对TK基因转染的淋巴瘤细胞的抑制作用 |
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| 引用本文： | 吕鸣,傅卫军,侯健,王东星,丁思奇.更昔洛韦对TK基因转染的淋巴瘤细胞的抑制作用[J].武警医学,2003,14(11):652-654. |
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| 作者姓名： | 吕鸣 傅卫军 侯健 王东星 丁思奇 |
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| 作者单位： | 武警上海总队医院血液科,上海,201103;第二军医大学长征医院血液科,上海,200003 |
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| 摘 要： | 目的探讨GCV对TK基因转染的淋巴瘤细胞生长的抑制作用.方法利用缺陷型逆转录病毒介导的TK基因(RV-HSV-TK)转染淋巴瘤细胞,细胞增殖实验(MTT法),研究GCV对转染TK基因阳性淋巴瘤细胞株杀伤作用.结果转染了TK基因的淋巴瘤细胞的生长曲线与未转染TK基因的细胞无差异.0.1～50μg/ml的GCV对转染阳性细胞有明显的毒性作用,杀伤效应与时间成正比.Hut-tk细胞对Hut细胞具有旁观者效应.结论RV-HSV-TK系统对淋巴瘤细胞有较高的转染效率,转染了TK基因的淋巴瘤细胞对GCV敏感.
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| 关 键 词： | TK基因 淋巴瘤 基因治疗 |
| 收稿时间： | 2003-08-11 |
| 修稿时间： | 2003年8月11日 |
Selective killing of TK gene transfected lymphoma cell lines by ganciclovir |
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| Lu Ming,FU Weijun,HOU Jian,WANG Dongxing,and DING Siqi.Selective killing of TK gene transfected lymphoma cell lines by ganciclovir[J].Medical Journal of the Chinese People's Armed Police Forces,2003,14(11):652-654. |
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| Authors: | Lu Ming FU Weijun HOU Jian WANG Dongxing and DING Siqi |
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| Institution: | Lu Ming,FU Weijun,HOU Jian,WANG Dongxing,and DING Siqi.Department of Hematology,Shanghai Municipal Corps Hospital,Chinese People's Armed Police Forces,Shanghai 201103,China |
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| Abstract: | Objective To study the inhibitory effect of ganciclovir(GCV) on lymphoma cells transfected with TK gene. Methods By using RV-HSV-TK gene system and MTT technique, the growth inhibiting effect of GCV on lymphoma cells tramsfected with TK gene was analysed . Results Herpes TK gene was successfully transmitted into lymphoma cell lines by retrovirus. GCV in concentrations ranging from 0.1 to 50ug/ml showed significant toxicity on lymphoma cells with TK gene; the effect was time dependent. By-slander effect was observed after coin-cubation of Hut-tk cells with Hut cells. Conclusions RV-HSV-TK system has high efficiency in transfecting lymphoma cells with TK gene by mediation of retrovirus,and these cells are sensitive to GCV. |
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| Keywords: | TK gene Lymphoma Gene therapy |
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