转录因子T-bet在变应性鼻炎患者鼻咽相关淋巴组织中的表达特征和意义

目的:探讨T-bet在变应性鼻炎(AR)和非变应性鼻炎(NAR)2组人群的鼻咽相关淋巴组s织(NALT)中的表达特征和意义。方法:分别用免疫组织化学单染色法检测AR患者和NAR人群扁桃体、腺样体和鼻黏膜中的T-bet阳性表达,用免疫组织化学双染色法检测CD4/T-bet表达。结果:在AR组扁桃体、腺样体和鼻黏膜组织中T-bet表达阳性率高于NAR组,差异有统计学意义(均P<0.05);AR组不同年龄之间扁桃体T-bet表达存在差异(P<0.05),其中主要是成年人T-bet表达低于儿童和青少年;在NAR组扁桃体组织T-bet表达没有年龄之间的差异。2组中扁桃体、腺样体和鼻黏膜中均有CD4和T-bet表达,大多数T-bet阳性表达位于CD4 T细胞中。结论:在AR患者NALT中T-bet的下调表达,与AR患者呼吸道局部黏膜免疫转录因子水平的Th1反应低下有关。NALT中的T-bet表达既与黏膜的变应性炎症有关,又与NALT的功能状态有关。

Expression of T-bet in Nasopharyngeal associated lymphatic tissue of patients with allergic rhinitis

Objective:To investigate the pattern and value of the expression of T-bet in nasopharyngeal-associated lymphatic tissue(NALT) of patients with or without allergic rhinitis(AR).Method:T-bet and CD4/T-bet expression in adenoids,tonsils and nasal mucosa was respectively detected with single stain and double stain of immunohistochemistry.Result:The positive ratio of T-bet expression in tonsils,adenoids and nasal mucosa between AR group and the control group had statistically different(P<0.05).There was statistical difference of T-bet expression in tonsils from AR group in three different age groups(P<0.05).The difference was that T-bet expression in adults group was significantly lower than that in children group and adolescent group.There was no difference of T-bet expression among different age groups in non-AR group.There were some positive expression of CD4 and T-bet in tonsils,adenoids and nasal mucosa from two groups and most positive expression of T-bet on CD4 positive cell.Conclusion:T-bet expression was down-regulated in NALT of patients with AR.T-bet expression of NALT is associated with allergic mucosal inflammation and functional status of NALT,as well as the weak Th1 response at the level of transfer factor in local mucosa of respiratory tract in AR patients.