非小细胞肺癌中肿瘤微环境细胞的表达特点及其与临床生存概率的相关性

目的:探究常见的肿瘤微环境细胞在非小细胞肺癌中的表达特点及其与临床生存概率的相关性。方法:我们首先从TCGA数据库中获取肺腺癌、肺鳞癌的基因表达数据和相应的临床信息；接着通过非参数检验分析了几种常见肿瘤微环境细胞在癌组织和癌旁肺组织之间的表达差异，以及细胞浸润程度与非小细胞肺癌生存概率之间的关系,并进行了多因素生存分析。结果:在肺腺癌中，CD4+、CD8+T细胞、内皮细胞、巨噬细胞低表达，B细胞、肿瘤相关成纤维细胞（cancer associated fibroblasts，CAFs）高表达，生存概率与CD4+T细胞呈正相关；在肺鳞癌中，内皮细胞、巨噬细胞低表达，CAFs高表达，生存概率与CD8+T细胞呈正相关。结论:在肺腺癌、肺鳞癌肿瘤微环境中，浸润细胞的表达量各不相同，且与临床生存概率相关。

Investigation of expression profiles of tumour microenvironment cells and the correlation with clinical survival probability in non-small cell lung cancer

Objective:To explore the expression profiles of tumor microenvironment cells in non-small cell lung cancer and the correlation with clinical survival probability.Methods:Firstly,we obtained gene expression data and relevant clinical information of lung adenocarcinoma and lung squamous cell carcinoma from the TCGA database.Then,the expression differences of several common tumor microenvironment cells between cancer tissues and adjacent lung cancer tissues were analyzed by non-parametric test,as well as the relationship between the degree of cell invasion and the survival probability of non-small cell lung cancer,and the multi-factor survival analysis was carried out.Results:In lung adenocarcinoma,the expressions of CD4~+,CD8~+T cells,endothelial cells and macrophages were low,while those of B cells and CAFs cells were high.The survival probability was positively correlated with CD4~+T cells.In lung squamous cell carcinoma,the expression of endothelial cells and macrophages was low,and the expression of CAFs was high.The survival probability was positively correlated with CD8~+T cells.Conclusion:The expression of infiltrating cells is different in the tumor microenvironment of lung adenocarcinoma and lung squamous cell carcinoma,and it is related to the clinical survival probability.