miR-449b表达与原发性肝细胞癌预后的关系

目的 探讨原发性肝细胞癌患者外周血中微小RNA(miR)-49b的表达水平及其与预后的关系.方法 选取2013年1月至2015年1月行根治切除术的98例原发性肝细胞癌患者作为观察组,选取同期30例健康体检者作为对照组.用实时荧光定量聚合酶链反应(qRT-PCR)检测研究对象外周血中miR-449b的表达水平.对观察组患...

Relationship between miR-449b expression and prognosis of primary hepatocellular carcinoma

Objective To investigate the expression level of microRNA(miR)-449 b in peripheral blood and its relationship with prognosis of patients with primary hepatocellular carcinoma(PHC).Methods A total of 98 PHC patients undergoing radical resection from January 2013 to January 2015 were selected as observation group,and 30 healthy examined people during the same period were served as control group.The quantitative real time polymerase chain reaction assay was used to detect the expression level of miR-449 b in peripheral blood.The patients in observation group were followed up until October 2019 to observe the overall survival(OS)and analyze the association of miR-449 b expression with OS.Results The expression level of miR-449 b in observation group was significantly lower than that in control group(0.25±0.09 vs 0.59±0.12,t=16.674,P<0.01).In the 90 followed patients,49 patients died.Kaplan-Meier combined with Log-rank test showed that OS in patients with low expression of miR-449 b were significantly shorter than that in patients with high expression of miR-449 b(P<0.05).Cox analysis showed that lower miR-449 b expression,older age and higher TNM stage were the independent risk factors for OS in PHC patients(P<0.05).Conclusion In patients with PHC,the down-regulation expression of miR-449 b is associated with poor OS,and peripheral blood detection of miR-449 b may be an effective prognostic marker of PHC.