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| Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells | |
| 引用本文： | 李新宇 李中国 邱良秀 赵长松 胡竹林. Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells[J]. 华中科技大学学报(医学英德文版), 2005, 25(5): 575-577. DOI: 10.1007/BF02896022 |
| 作者姓名： | 李新宇 李中国 邱良秀 赵长松 胡竹林 |
| 作者单位： | Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| 摘 要： | Summary： In order to investigate the effect of nerve growth factor （NGF） on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF.MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570 nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mI. and 500 U/mI. NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different. |
| 关 键 词： | 神经生长因子 细胞增殖 家兔 动物实验 角膜损伤 上皮细胞 |
| 收稿时间： | 2004-03-03 |
Nerve growth factor modulate proliferation of cultured rabbit corneal endothelial cells and epithelial cells |
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| Li Xinyu,Li Zhongguo,Qiu Liangxiu,Zhao Changsong,Hu Zhulin. Nerve growth factor modulate proliferation of cultured rabbit corneal endothelial cells and epithelial cells[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2005, 25(5): 575-577. DOI: 10.1007/BF02896022 | |
| Authors: | Li Xinyu Li Zhongguo Qiu Liangxiu Zhao Changsong Hu Zhulin |
| Affiliation: | Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| Abstract: | In order to investigate the effect of nerve growth factor (NGF) on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF. MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570 nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mL and 500 U/mL NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different. |
| Keywords: | nerve growth factor corneal endothelial cells corneal epithelial cells proliferation |
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