黑色素瘤中VEGF-C和微淋巴管密度检测及其临床意义

目的:探讨血管内皮生长因子C和微淋巴管密度在黑色素瘤组织中的表达及其临床意义?方法:免疫组化检测黑色素瘤中血管内皮生长因子-C(vascular endothelial growth factor C,VEGF-C)表达和D2-40标记的微淋巴管密度,分析VEGF-C和微淋巴管密度的相关性及两者与黑色素瘤临床病理特点的关系?生存分析评价两者表达高低对黑色素瘤无转移生存率的影响?取黑痣为对照?结果:黑色素瘤微淋巴管密度(14.30±4.91)明显高于黑痣(7.64±3.08)(P < 0.01);黑色素瘤溃疡组的微淋巴管密度高于无溃疡组(P=0.005);VEGF-C表达和临床病理特征未发现相关性(P > 0.05),且其表达强度高低对微淋巴管密度大小及无转移生存率均无影响(P > 0.05)?而微淋巴管密度≥14.7比<14.7的黑色素瘤患者的无转移生存率缩短(P < 0.05)?结论:黑色素瘤内部存在新生淋巴管,微淋巴管密度大小和原发灶有无溃疡正相关,检测微淋巴管密度可以预测黑色素瘤淋巴结转移?

Clinical significance of VEGF-C expression and lymphatic micro-vessel density in melanoma

Objective:To explore the expression of vascular endothelial growth factor C(VEGF-C) and lymphatic micro-vessel density in their clinical significance of melanoma tissues. Methods:The expression of VEGF-C and D2-40 labeled lymphatic micro-vessel density in melanoma were determined by immunohistochemistry. Both the correlation between VEGF-C and lymphatic micro-vessel density and the relation of their expression with clinical and pathological features of melanoma were analyzed. Then their effect on metastasis-free survival rate in melanoma were separately evaluated by survival analysis. Patients with nevi were used as control. Results:The lymphatic micro-vessel density in melanoma(14.30±4.91)was more than that in the nevi(7.64±3.08)(P < 0.01). The lymphatic micro-vessel density were higher in ulcer group than in non-ulcer group(P=0.005).There was no significant correlation between VEGF-C expression and clinicopathological characteristics(P > 0.05). The intensity of VEGF-C expression has no effect on lymphatic micro-vessel density and metastasis-free survival rate(P > 0.05). The metastasis-free survival rate in patients with melanoma,whose lymphatic micro-vessel density is more than or equal to 14.7,was significantly shorter than those less than 14.7. Conclusion:Lymphatic vessels were found within tumor. The lymphatic micro-vessel density is positively correlated with ulcer,and can predict lymph node metastasis of melanoma.