透明质酸钠复合rhBMP-2促进骨质疏松症种植体骨结合的实验研究

目的:探讨重组人骨形成蛋白-2(rhBMP-2)对于骨质疏松症种植体骨结合的影响?方法:构建骨质疏松症大鼠模型,随机分为对照组?模型组和实验组,于胫骨内植入种植体?实验组种植体周围植入透明质酸钠复合rhBMP-2,对照组及模型组植入同等体积的透明质酸钠?术后4周和8周,通过X线?组织学切片及扫描电镜分析技术,观察各组胫骨内种植体骨结合的情况?结果:实验组种植体周围新生骨组织包绕,骨质致密,结构优良,未见明显骨吸收现象,骨结合良好?对照组种植体周围同样新生骨形成,部分区域见炎症反应,少量结缔组织长入种植体周围?模型组种植体周围骨组织形成较少,种植体脱落,未形成骨结合,种植体窝内炎症反应明显,周围结缔组织增生,部分区域出现骨质断裂?结论:rhBMP-2能够显著促进骨质疏松症大鼠胫骨内种植体周围新骨形成,加快成骨速度,促进骨质矿化,形成良好的骨结合?

The effect of pre-implant osteointegration with recombinant human bone morphogenetic protein-2 in ovariectomized rats

Objective:To investigate the effects of recombinant human bone morphogenetic protein-2 on osteointegration of implant in ovariectomized rats. Methods:Thirty old femal Wistar rats were randomly divided into three groups:Sham-OVX,OVX and OVX+rhBMP-2. One implant was inserted in the right tibia of each rat. Meanwhile,rhBMP-2 and 1% SA composite were embedded in the peri-implant area in OVX+rhBMP-2 group,and 1% SA was embedded in Sham-OVX and OVX groups. The bone density of right tibia was observed with X-ray and the serum ALP activity was measured pre- and post ovariectomy using a ALP-kit. Four- and eight-week after implantation,the rats were sacrificed and the right tibias with implant were taken by X-ray. History changes were observed by HE stain and SEM scanning. Results:New bone formation was determined in the peri-implant area with good osseointegration in the OVX+rhBMP-2 and Sham-OVX group. No bone absorption was observed in the OVX+rhBMP-2 group,while some soft tissue was observed in the Sham-OVX group. In the OVX group,the implant was lost with severe inflammation and bone fracture in the normal bone tissue. Conclusion:rhBMP-2 could enhance bone osseointegration and induced new bone formation in ovariectomised rats.