Performance of a commercial, type-specific enzyme-linked immunosorbent assay for detection of herpes simplex virus type 2-specific antibodies in Ugandans |
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| Authors: | Laeyendecker Oliver Henson Charla Gray Ronald H Nguyen Ruby H-N Horne Bobbi Jo Wawer Maria J Serwadda David Kiwanuka Noah Morrow Rhoda Ashley Hogrefe Wayne Quinn Thomas C |
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| Affiliation: | National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA. |
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| Abstract: | ![]()
Two hundred forty-eight human immunodeficiency virus (HIV)-positive and 496 HIV-negative subjects in Uganda were tested by HerpeSelect herpes simplex virus type 2 enzyme-linked immunosorbent assay (ELISA) and Western blotting to optimize the ELISA for use in this population. A higher index cutoff value was required for optimal sensitivity and specificity, and overall performance of the assay was not affected by HIV status. |
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