三氧化二砷对大肠癌Ls174细胞致腹水生成的影响

目的研究三氧化二砷（As2O3）对人大肠癌裸鼠腹腔种植腹水生成的影响。方法将大肠癌Ls174细胞通过单独培养,与5-氟尿嘧啶（5-Fu）、As2O3分别共培养后行腹腔内注射构建裸鼠腹水模型,通过单独和联合注入化疗药物,观察腹水生成情况以及检测腹水细胞中耐药基因谷胱甘肽硫转移酶酸性同工酶（GST。百）mRNA表达,比较三氧化二砷对大肠癌致腹水生成的影响。结果5FU和As2O3组裸鼠腹水生成明显减少,且生存期延长,耐药基因GST-π TmRNA表达最低。结论三氧化二砷能够抑制或消除人大肠癌裸鼠腹腔种植及腹水的生成,不同程度地延长生存期并通过下调耐药基因GST-πmRNA表达提高对化疗药物的敏感性。

Effect of arsenic trioxide on ascetic production in human colon cancer cell line Ls174

Objective To investigate the effect of arsenic trioxide on ascetic production in human colon cancer cell line Ls174.Methods Three kinds of ascites tumor-bearing mice model were constructed through intraperitoneal injection with Lsl74 or well-prepared Ls174 incubated with 5-FU or As_2O_3.The effect of As_2O_3 on ascetic production was evaluated for every ascites tumor-bearing mice model,mice received 5-FU,As_2O_3,or As_2O_3 combined with 5-FU intraperitoneally, respectively.The ascetic productiogroln and survival time was observed.Meanwhile,the expression of glutathione-s-transferase π(GST-π)in ascites was measured by RT-PCR.Results The prodwtion of ascites in group As_2O_3 with 5-FU is significatly inhibited,and the survival period is prolonged whaty more,the expression of GST-πmRNA was significant down.Conclusion As_2O_3 combined with 5-FU can inhibit and eradicate production of ascites and prolong the survival period of nude mice.As_2O_3 is able to partly reverse the resistance of the Lsl74 cell of human cancer of colon.which may be related to the variation of GST-π.