| 磺酰罗丹明法检测多巴胺能神经细胞的条件 |
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| 引用本文： | 张乃钲,王艳秋,徐胜利,陈彪.磺酰罗丹明法检测多巴胺能神经细胞的条件[J].中国行为医学科学,2005,14(1):92-94. |
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| 作者姓名： | 张乃钲 王艳秋 徐胜利 陈彪 |
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| 作者单位： | 首都医科大学宣武医院老年中心，国家人类基因组北方研究中心，首都医科大学宣武医院老年中心，首都医科大学宣武医院老年中心 100053 北京，100053 北京，100053 北京 |
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| 基金项目： | 国家高技术研究发展计划(863)计划项目(2002AA2Z2011)
国家自然科学基金资助项目(30371574)
北京市卫生局首批重点学科项目北京市自然科学基金重点项目 |
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| 摘 要： | 目的探讨磺酰罗丹明B法检测多巴胺能神经细胞的最佳条件。方法采用系列细胞密度梯度、选用多个波长、加用参考波长,以磺酰罗丹明B法检测多巴胺能细胞系Mes23.5细胞数量和活性,分析细胞数量与检测波长及吸光度的关系。结果细胞数量与吸光度呈线性关系,最佳检测波长为540nm。结论磺酰罗丹明B法检测多巴胺能细胞结果可靠,是切实可行的该细胞定量分析和活性检测方法。
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| 关 键 词： | 磺酰罗丹明B法 细胞数量 吸光度 多巴胺能神经细胞 最佳条件 |
| 修稿时间： | 2004年5月23日 |
Studv on detecting conditions of the dopaminergic neural cell by SRB assay |
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| Zhang Nai-zheng,WangYan-qiu,Xusheng-li,el at. Senile Disease Center,Xuanwu Hospital,Capital University of Medical Science,Beijing ,China.Studv on detecting conditions of the dopaminergic neural cell by SRB assay[J].Chinese Journal of Behavioral Medical Science,2005,14(1):92-94. |
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| Authors: | Zhang Nai-zheng WangYan-qiu Xusheng-li el at Senile Disease Center Xuanwu Hospital Capital University of Medical Science Beijing China |
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| Institution: | Zhang Nai-zheng,WangYan-qiu,Xusheng-li,el at. Senile Disease Center,Xuanwu Hospital,Capital University of Medical Science,Beijing 100053,China |
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| Abstract: | Objective To explore the optimal detecting conditions of the dopaminergic neural cell by SRB assay. Methods A series of cell density grads, sereval working wavelengths, and reference wavelengths were chosen to detect the number and the activity of the dopaminergic neural cell Mes23. 5 by SRB assay. The relations between cell number and the absorbency were analysed. Results The relations between cell number and the absorb-ency were linear, and the optimal working wavelength was 540nm. . Conclusion The detecting result of the dopaminergic neural cell by SRB assay is reliable, so SRB assay is a practical method to quantitatively analyse the dopaminergic neural cell and detect their activity. |
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| Keywords: | SRB assay Cell count Absorbency Dopaminergic neural cell Optimal detecting conditions |
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