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| 原核表达HPV16 L1蛋白ELISA方法检测血清抗体 | |
| 引用本文： | 赵丽晶,王学林,许多,梁作文,郭恒,孙树民,赵淑华,赵雪俭,郑晶莹.原核表达HPV16 L1蛋白ELISA方法检测血清抗体[J].中国妇幼保健,2010,25(3). |
| 作者姓名： | 赵丽晶 王学林 许多 梁作文 郭恒 孙树民 赵淑华 赵雪俭 郑晶莹 |
| 作者单位： | 1. 吉林大学基础医学院病理生理学教研室,吉林,长春,130021 2. 吉林大学人兽共患病教育部重点实验室 3. 吉林大学第二医院妇产科 |
| 摘 要： | 目的:从感染人乳头瘤病毒(HPV)的新鲜宫颈癌组织中扩增HPV16型晚期蛋白L1基因全长,构建原核表达载体,表达并纯化蛋白用于ELISA检测。方法:根据基因序列设计一对特异引物,用PCR的方法从宫颈癌组织DNA中获得L1基因,以pet28a为载体构建表达质粒,IPTG诱导表达蛋白,DEAE及葡聚糖凝胶分离纯化目的蛋白,将蛋白包被平底96孔板用于ELISA检测。结果:从宫颈癌临床标本克隆到HPV16型L1蛋白编码序列,其原核表达产物纯化后可用于ELISA检测。结论:成功获得人有抗原性的HPV16L1蛋白,可用于ELISA检测。 |
| 关 键 词： | HPV16 L1蛋白 原核表达 ELISA检测 |
ELISA detection of prokaryotic expression serum antibody of HPV16 L1 protein |
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| ZHAO Li-Jing,WANG Xue-Lin,XU Duo et al.ELISA detection of prokaryotic expression serum antibody of HPV16 L1 protein[J].Maternal and Child Health Care of China,2010,25(3). | |
| Authors: | ZHAO Li-Jing WANG Xue-Lin XU Duo |
| Institution: | ZHAO Li-Jing,WANG Xue-Lin,XU Duo et al.Department of Pathophysiology,College of Preclinical Medicine,Jilin University,Changchun 130021,Jilin,China |
| Abstract: | Objective:To amplify the HPV16 late protein L1 gene in fresh cervical cancer tissues infected by human papillomavirus (HPV),construct prokaryotic expression vector,then the protein was expressed,purified and used for ELISA detection.Methods:Specific primers were designed according to gene sequence.L1 gene was obtained from cervical cancer tissues DNA by PCR to construct recombinant prokaryotic expression vector pet28a-HPV16-L1.L1 protein expression was induced by IPTG,purified by DEAE and sephadex,and packa... |
| Keywords: | HPV16L1 protein Prokaryotic expression ELISA detection |
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