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| Chemical synthesis and cloning of human calcitonin (hCT) cDNA | |
| 引用本文： | 蔡在龙,窦鸿,毛积芳,邹鲁峰,伍军,王官将. Chemical synthesis and cloning of human calcitonin (hCT) cDNA[J]. 中国人民解放军军医大学学报, 1997, 0(3) |
| 作者姓名： | 蔡在龙 窦鸿 毛积芳 邹鲁峰 伍军 王官将 |
| 作者单位： | Department of Biochemistry，Second Military Medical University，Shanghai，200433 |
| 摘 要： | Humancalcitonin(hCT)isanoligo--peptidehormonecontaining32aminoacidresidues(aa)whichisproducedbytheC--parafollicularcellsofthethyroidglandinhumans.Togetherwiththearathyoidichormoneand1,25--dihydroxycholecalciferol,hCTplaysanimportantroleintheregulationofcalciumandphosphatemetabolism.Itstimulatestheimmobilizationofthecalciuminthebonesystem,preventingtheresorptionofthebones.ThehCThasbeenadministeredfortreatmentofpatientssufferingfromvariousdiseaseswherestronghypercalcemiaorboneresorptionoccurs… |
Chemical synthesis and cloning of human calcitonin (hCT) cDNA |
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| Cai Zailong, Dou Hong, Mao Jifang, Zou Lufeng,Wu Jun, Wang Guanjiang. Chemical synthesis and cloning of human calcitonin (hCT) cDNA[J]. Journal of Medical Colleges of PLA(China), 1997, 0(3) | |
| Authors: | Cai Zailong Dou Hong Mao Jifang Zou Lufeng Wu Jun Wang Guanjiang |
| Abstract: | Chemical synthesis and cloning of human calcitonin (hCT) cDNA. Methods and Results:The cDNA sequence coding for human calcitonin (hCT) was divided into 6 fragments, synthesized by ABI 391 DNA synthesis instrument and cloned into PGEM7Z( ) vector following phosphorylation, annealing and ligation of the 6 fragments. The sequence of cloned hCT cDNA was proved to be the same as designed by double DNA sequencing. Conclusion: A reliable and forthright method of chemical synthesis and cloning of oligo-peplides cDNA (or gene) was provided in this study. This paper is a basic work for expression of hCT cDNA. |
| Keywords: | human calcitonin cDNA chemical synthesis cloning |
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