塞来昔布对人成骨细胞增殖的影响及其作用机制

目的：观察塞来昔布对人成骨细胞增殖的影响及其作用机制。方法将人成骨细胞分为3个组：空白对照组、IL-1刺激组(阴性对照组)、塞来昔布+ IL-1刺激组(药物干预组)，用 RT-PCR 法和 Western blot 法分别检测三组成骨细胞环氧化酶-2( COX-2)、膜相关前列腺素 E2合成酶1(mPGES-1) mRNA 和蛋白的表达。 MTT 法检测塞来昔布对阴性对照组人成骨细胞增殖的影响。结果 RT-PCR 法和Western blot 法实验结果显示，同空白对照组相比，阴性对照组的人成骨细胞中 COX-2、mPGES-1 mRNA 和蛋白的表达均增加；同阴性对照组相比，药物干预组的 COX-2、mPGES-1 mRNA 和蛋白的表达均降低。塞来昔布对 IL-1刺激的人成骨细胞的生长具有抑制作用，呈现剂量依赖性，浓度越大，抑制作用越大；并且随着作用时间的延长，细胞的抑制作用增大。结论塞来昔布可以抑制炎性状态下人成骨细胞的生长，可能是通过调控 COX-2、mPGES-1 mRNA 和蛋白的表达，使其表达下调而发挥作用。

Study on the impact of celecoxib on proliferation of human osteoblasts

Objective To study the impact of celecoxib on proliferation of human osteoblasts. Methods The hu-man osteoblasts were cultured and divided into three groups,including the negative control, positive control (stimu-lated by IL-1) and intervention group (celecoxib + IL-1). The detection of mRNA and protein level of COX-2 and mPGES-1 were measured by real tmie PCR (RT-PCR) and Western blot. MTT assay was performed to determine the impact of celecoxib on proliferation of human osteoblasts which were treated with IL-1. Results RT-PCR and Western blot showed that the detection of mRNA and protein level of COX-2 and mPGES-1 of the positive control (stimulated by IL-1) were significantly higher than that of the negative control and intervention group (celecoxib +IL-1). Celecoxib had cytotoxic effect on the growth of osteoblasts, manifests dose dependence as well as increasing cell inhibition over time. Conclusion Celecoxib could inhibit proliferation of osteoblasts, and could inhibit the oc-currence of heterotopic ossification by regulating the level of mPGES-1 and COX-2 mRNA and protein.