| 高效液相色谱法测定雄芍颗粒中芍药苷的含量 |
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| 引用本文： | 梁惠珍,李霞,门九章,张海春. 高效液相色谱法测定雄芍颗粒中芍药苷的含量[J]. 山西中医, 2014, 0(9): 48-49 |
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| 作者姓名： | 梁惠珍 李霞 门九章 张海春 |
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| 作者单位： | 山西中医学院,太原030024 |
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| 基金项目： | 山西省太原市大学生创新创业专题项目（编号：100115176）. |
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| 摘 要： | 目的:采用高效液相色谱法测定雄芍颗粒中芍药苷的含量。方法:采用HPLC法,色谱柱:Diamonsil C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液(14∶86);流速:1.0 mL/min;检测波长:230 nm。结果:芍药苷在0.107 8~2.156 0μg线性关系良好(r=0.999 7);平均回收率为99.26%,RSD为2.06%(n=5)。结论:该方法简便、准确、可靠、重现性好,可用于雄芍颗粒的质量控制。
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| 关 键 词： | 雄芍颗粒 白芍 芍药苷 高效液相色谱法 |
Determination of Paeoniflorin in Xiongshao Granule by HPLC |
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| Affiliation: | LIANG Hui-zhen, LI Xia, MEN Jiu-zhang, et al. ( Shanxi College of Traditional Chinese Medicine, Taiyuan, 030024, China) |
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| Abstract: | Objective： To determine Paeoniflorin in Xiongshao Granule by HPLC. Method： HPLC was used in the quantitative analysis of Paeoniflorin in the sample. The separation was performed on Diamonsil C18 （250 mm× 4.6 mm,5μm） column with a mixture of acetonitrile 0. 1% phosphoric acid （ 14：86） as mobile phase. The flow rate was 1.0 mL/min and the detection wavelength was set at 230 nm. Result： The linear range of paeoniflorin was 0. 107 8 -2. 156 0 μg （ r = 0. 999 7 ）, the average recovery was 99.26%, RSD = 2. 06% （ n = 5 ）. Conclusion： The method is simple, accurate, reliable, good reproductive and could be used for the quality control of paeoniflorin in Xiongshao granule. |
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| Keywords: | xiongshao granule paeonia lactiflora paeoniflorin HPLC |
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