| 人脐带血与周围血中NKT细胞频率、亚群、表型及功能的比较 |
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| 引用本文： | 李子涛,杨滨燕,刘昀,王慧,张建平,陈慧,吴长有. 人脐带血与周围血中NKT细胞频率、亚群、表型及功能的比较[J]. 免疫学杂志, 2011, 0(4): 277-282 |
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| 作者姓名： | 李子涛 杨滨燕 刘昀 王慧 张建平 陈慧 吴长有 |
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| 作者单位： | 中山大学中山医学院免疫学研究所热带传染病教育部重点实验室; |
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| 摘 要： | ![]()
目的比较足月产新生儿脐带血和正常人周围血NKT细胞的频率、亚群、表型特征及功能。方法分离足月产新生儿脐带血CBMCs和正常成年人周围血PBMCs,流式细胞术检测TCRvβ11、CD4、CD8、CD45RA、CD62L、CCR7等表面分子的表达及细胞因子IL-4和IFN-γ的产生。结果 CBMCs和PBMCs中CD3+TCRvβ11+NKT细胞的平均频率分别为0.35%和0.33%,二者无显著差别。CBMCs中CD4+NKT细胞频率(67.39%)高于PBMCs中CD4+NKT细胞频率(54.08%),但CD8+NKT细胞,PBMCs明显高于CBMCs(22.35%对5.86%),CD4-CD8-NKT二者相差无统计学意义。CBMCs中CD3+TCRvβ11+CD45RA+NKT细胞的频率(88.37%)高于PBMCs(61.32%)。CBMCs中CD62L(56.66%对49.60%)和CCR7(22.64%对20.03%)的比例稍高于PBMCs,但其差别无显著性。未刺激CBMCs和PBMCs中CD3+TCRvβ11+NKT细胞均不产生IL-4和IFN-γ,PMA+Ionomycin刺激后,CBMCs中CD3+TCRvβ11+NKT细胞仍不能产生IL-4和IFN-γ,但PBMCs中CD3+TCRvβ11+NKT细胞却有6.74%产生IL-4、26.96%产生IFN-γ、1.26%同时分泌IL-4和IFN-γ,产生细胞因子的CD3+TCRvβ11+NKT细胞主要为记忆性NKT细胞。结论 CBMCs和PBMCs中CD3+TCRvβ11+NKT细胞的亚群、表型和功能均存在一定差别,记忆性NKT细胞可能与其再次免疫应答相关。
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| 关 键 词： | NKT细胞 频率 亚群 表型 细胞因子 |
Comparison of frequencies,subpopulations,phenotypes,biological functions of human NKT cells from cord blood mononuclear cells and peripheral blood mononuclear cells |
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| LI Zitao,YANG Binyan,LIU Yun,WANG Hui,ZHANG Jianping,CHEN Hui,WU Changyou Key Laboratory of Tropical Disease Control Research of Ministry of Education. Comparison of frequencies,subpopulations,phenotypes,biological functions of human NKT cells from cord blood mononuclear cells and peripheral blood mononuclear cells[J]. Immunological Journal, 2011, 0(4): 277-282 |
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| Authors: | LI Zitao YANG Binyan LIU Yun WANG Hui ZHANG Jianping CHEN Hui WU Changyou Key Laboratory of Tropical Disease Control Research of Ministry of Education |
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| Affiliation: | LI Zitao,YANG Binyan,LIU Yun,WANG Hui,ZHANG Jianping,CHEN Hui,WU Changyou Key Laboratory of Tropical Disease Control Research of Ministry of Education,Institute of Immunology,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China |
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| Abstract: | ![]()
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| Keywords: | NKT cells Frequency Subpopulation Phenotype Cytokine |
| 本文献已被 CNKI 等数据库收录！ |
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