| 日本血吸虫模拟虫卵抗原的初步研究 |
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| 引用本文： | 钱旻,白艳军,章平,吴自荣,严自助. 日本血吸虫模拟虫卵抗原的初步研究[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(5): 312-313 |
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| 作者姓名： | 钱旻 白艳军 章平 吴自荣 严自助 |
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| 作者单位： | 1. 华东师范大学生物系,上海,200062
2. 中国预防医学科学院寄生虫病研究所,上海,200025 |
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| 基金项目： | 国家"九五"攻关项目资助(No.96-906-04-04)Supported by the Ninth Five-year Key Research Project(No. 96-906-04-04) |
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| 摘 要： | 日本血吸虫虫卵是主要的致病因子 ,但虫卵抗原的制备比较繁琐 ,需要大量的实验动物。为了探索和发展可用于诊断和疫苗的模拟虫卵抗原 ,我们采用噬菌体展示随机 15 肽文库对日本血吸虫虫卵单克隆抗体 6Ｂ12所识别的抗原表位进行了模拟。用 6Ｂ12作为“诱饵” ,采用生物淘金法通过 3轮的筛选使得与 6Ｂ12有高亲和力的噬菌体得到有效的富集。随后采用ＥＬＩＳＡ、竞争ＥＬＩＳＡ、Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ等检测方法 ,从 40 0个单克隆化的噬菌体株中得到了 13株与 6Ｂ12有特异性、高亲和力反应的噬菌体克隆。经过对噬菌体所携带的外源…
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| 关 键 词： | 日本血吸虫 模拟 虫卵抗原 初步研究 |
| 文章编号： | 1000-7423(2000)-05-0312-02 |
| 修稿时间： | 1999-12-28 |
PRELIMINARY STUDY ON SCREENING OF EGG MIMOTOPE OF SCHISTOSOMA JAPONICUM |
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| QIAN Min,BAI Yan-jun,ZHANG Ping,WU Zi-rong,YAN Zi-zhu. PRELIMINARY STUDY ON SCREENING OF EGG MIMOTOPE OF SCHISTOSOMA JAPONICUM[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2000, 18(5): 312-313 |
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| Authors: | QIAN Min BAI Yan-jun ZHANG Ping WU Zi-rong YAN Zi-zhu |
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| Affiliation: | Department of Biology, East China Normal University, Shanghai 200062. |
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| Abstract: | The egg of Schistosoma japonicum is a chief pathogenic factor. The preparation of egg antigen is a troublesome work, and needs a large number of experimental animals. In order to explore and develop an egg mimotope that can be used in the study of diagnostic reagents and vaccines, we employed the phage display random 15-peptides library to mimic the antigen epitope recognized by mAb 6B12 which is specific to egg antigen of Schistosoma japonicum. |
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