| 不同大小机械牵张力对MC3T3-E1成骨样细胞MMP-13/TIMP-1 mRNA表达的影响 |
| |
| 引用本文： | 唐林,林珠,李永明.不同大小机械牵张力对MC3T3-E1成骨样细胞MMP-13/TIMP-1 mRNA表达的影响[J].中国美容医学,2006,15(4):361-363. |
| |
| 作者姓名： | 唐林 林珠 李永明 |
| |
| 作者单位： | 第四军医大学口腔医学院正畸科,陕西,西安,710032 |
| |
| 摘 要： | 目的:研究不同大小的机械牵张力对成骨细胞MMP-13/TIMP-1mRNA表达的影响。方法:通过自制的多通道细胞牵张应力加载系统对小鼠成骨样细胞MC3T3-E1同时施加6%、12%和18%的机械牵张力,作用24h后,用RT-PCR方法检测细胞受力后MMP-13/TIMP-1mRNA表达的变化。结果:细胞受力后,其MMP-13/TIMP-1mRNA表达随牵张力值的增大明显增加。结论:不同大小的机械牵张力可以影响成骨细胞的MMP-13/TIMP-1mRNA表达,进而影响骨改建。
|
| 关 键 词： | 机械牵张力 成骨细胞 MMP-13/TIMP-1 骨改建 |
| 文章编号： | 1008-6455（2006）04-0361-03 |
| 收稿时间： | 2006-01-14 |
| 修稿时间： | 2006-03-02 |
Effects of different magnitudes of mechanical strain on expression of MMP-13/TIMP-1mRNA in mouse osteoblast-like cells(MC3T3- E1) in vitro |
| |
| TANG Lin,LIN Zhu,LI Yong-ming.Effects of different magnitudes of mechanical strain on expression of MMP-13/TIMP-1mRNA in mouse osteoblast-like cells(MC3T3- E1) in vitro[J].Chinese Journal of Aesthetic Medicine,2006,15(4):361-363. |
| |
| Authors: | TANG Lin LIN Zhu LI Yong-ming |
| |
| Abstract: | Objective To investigate the effects of different magnitudes of mechanical strain on expression of MMP-13/TIMP-1 mRNA in mouse osteoblast-like cells(MC3T3-E1) in vitro.Methods MC3T3-E1 cells were subjected to 0%,6%,12%,18% elongation by using multi-passage cell stress loading system simultaneously for 24h,then MMP-13/TIMP-1 mRNA in MC3T3-E1cells were detected by RT-PCR.Results Expression of MMP-13/TIMP-1 mRNA in mouse osteoblast-like cells(MC3T3-E1) significantly increased in groups under strain(6%,12%,18% elongation) in magnitude-dependent manner compared to control after mechanical strain treatment for 24h.Conclusion Different magnitudes of mechanical strain can affect expression of MMP-13/TIMP-1 mRNA in MC3T3-E1 cells in magnitude-dependent manner,which deeply affects bone remodeling. |
| |
| Keywords: | mechanical strain osteoblasts MMP-13/TIMP-1 bone remodeling |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
