| PTD-BDNF对大鼠局灶性脑缺血损伤的保护作用 |
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| 引用本文： | 李冠华,冯泽国,周建平.PTD-BDNF对大鼠局灶性脑缺血损伤的保护作用[J].军医进修学院学报,2009,30(4):522-524. |
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| 作者姓名： | 李冠华 冯泽国 周建平 |
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| 作者单位： | 1. 解放军总医院麻醉手术中心,北京,100853
2. 军事医学科学院毒物药物研究所,北京,100850 |
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| 摘 要： | 目的观察PTD—BDNF在实验动物体内对脑缺血再灌注损伤的治疗效果。方法线栓法建立大鼠大脑中动脉闭塞模型。PTD—BDNF组于缺血再灌注后静脉注射PTD—BDNF 2mg／kg，对照组注射等体积生理盐水。采用TTC染色、HE染色及TUNNEL染色分析PTD—BDNF对脑缺血再灌注损伤的神经保护作用。结果在大鼠局灶性脑缺血再灌注模型中，与对照组相比，PTD—BDNF融合蛋白使脑缺血梗死体积百分比从18．20％降至10．12％（n=4，P〈0．01）。HE染色可见PTD—BDNF组脑组织缺血坏死程度明显减轻，细胞核皱缩、碎裂现象减少。TUNNEL染色显示梗死灶周边平均每个视野TUNNEL阳性细胞率，从51．24％降至23．54％（n=6，P〈0．01）。结论PTD—BDNF融合蛋白经静脉给药后能通过血脑屏障发挥神经保护作用，减轻脑缺血再灌注损伤，改善神经功能。
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| 关 键 词： | 大鼠 脑缺血 再灌注损伤 细胞凋亡 |
Protective effects of PTD-BDNF on cerebral ischemia injury in rats |
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| LI Guan-hua,FENG Ze-guo,ZHOU Jian-ping.Protective effects of PTD-BDNF on cerebral ischemia injury in rats[J].Academic Journal of Pla Postgraduate Medical School,2009,30(4):522-524. |
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| Authors: | LI Guan-hua FENG Ze-guo ZHOU Jian-ping |
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| Institution: | LI Guan-hua1,FENG Ze-guo1,ZHOU Jian-ping2 1Anesthesia , Operation Center,Chinese PLA General Hospital,Beijing 100853,China,2Institute of Pharmacology , Toxicology,Academy of Military Medical Sciences,Beijing 100850 |
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| Abstract: | Objective To observe the in vitro effects of PTD-BDNF on ischemia reperfusion injury in rats. MethodsA rat model of middle cerebral artery occlusion(MCAO) was established by intraluminal filament occlusion with thread ligation. Rats in PTD-BDNF group were intravenously injected with 2mg/kg PTD-BDNF immediately after ischemia reperfusion,and rats in control group were injected with the same volume of normal saline. TTC,HE and TUNEL staining were used to analyze the neuroprotective effect of PTD-BDNF on ische... |
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| Keywords: | rats brain ischemia reperfusion injury apoptosis |
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