|
|||||
|
|
| 消痰散结方对血管生成拟态相关蛋白表达的影响 | |
| 引用本文： | 周伟,李勇进,魏品康,陈天池,巨大维. 消痰散结方对血管生成拟态相关蛋白表达的影响[J]. 中国中医药信息杂志, 2011, 18(3): 44-46. DOI: 10.3969/j.issn.1005-5304.2011.03.016 |
| 作者姓名： | 周伟 李勇进 魏品康 陈天池 巨大维 |
| 作者单位： | 中国人民解放军第二军医大学长征医院中医科,上海,200003 |
| 摘 要： | 目的探讨消痰散结方对血管生成拟态（VM）相关蛋白表达的影响。方法30只裸鼠皮下移植人胃癌瘤块建立荷瘤模型。造模后的荷瘤鼠随机分为生理盐水组、消痰散结组、盐酸多西环素组，每组10只，分别给予相应药物灌胃治疗4周后，脱颈椎处死荷瘤鼠。观察移植瘤中VM的形态结构，计数VM的数量；运用免疫组织化学法检测肿瘤中基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）和层黏连蛋白5γ2链（LN-5γ2）等VM相关蛋白的表达。结果人胃癌裸鼠移植瘤可以形成VM。消痰散结组和盐酸多西环素组VM数量明显少于生理盐水组，差异有统计学意义（P〈0．01）。消痰散结组和盐酸多西环素组MMP-2、MMP-9和LN-5γ2蛋白的阳性表达低于生理盐水组，差异均有统计学意义（P〈0．01）。结论消痰散结方可抑制裸鼠移植瘤VM的形成，其机制可能与下调MMP-2、MMP-9和LN-5γ2蛋白表达有关。 |
| 关 键 词： | 消痰散结方 胃肿瘤 动物模型 血管生成拟态 基质金属蛋白酶 |
Effects of Xiaotan Sanjie Decoction on the Expression of Vasculogenic Mimicry Associated Proteins |
|
| ZHOU Wei,LI Yong-jin,WEI Pin-kang,CHEN Tian-chi,JU Da-wei. Effects of Xiaotan Sanjie Decoction on the Expression of Vasculogenic Mimicry Associated Proteins[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2011, 18(3): 44-46. DOI: 10.3969/j.issn.1005-5304.2011.03.016 | |
| Authors: | ZHOU Wei LI Yong-jin WEI Pin-kang CHEN Tian-chi JU Da-wei |
| Affiliation: | ZHOU Wei,LI Yong-jin,WEI Pin-kang,CHEN Tian-chi,JU Da-wei(Department of Traditional Chinese Medicine,Changzheng Hospital,Second Military Medical University of PLA,Shanghai 200003,China) |
| Abstract: | Objective To investigate the effects of Xiaotan Sanjie Decoction(XTSJD),a compound traditional Chinese herbal medicine,on the expression of vasculogenic mimicry(VM) associated proteins.Methods The tumor-bearing mice model was established by subcutaneously inoculating with xenografts of human gastric cancer into 30 BALB/c nude mice.After modeling,the tumor-bearing mice were randomly divided into normal saline(NS) group,XTSJD group and doxycycline hyclate(DH) group,10 mice in each group.And the mice were admi... |
| Keywords: | Xiaotan Sanjie Decoction stomach neoplasms animal model vasculogenic mimicry matrix metalloproteinases |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |