| 雷公藤红素对HMC-1细胞凋亡相关基因表达的影响 |
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| 引用本文： | 鲍一笑,张璐定,李莉,韩连书,孔宪涛. 雷公藤红素对HMC-1细胞凋亡相关基因表达的影响[J]. 第二军医大学学报, 2001, 22(9): 833-835 |
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| 作者姓名： | 鲍一笑 张璐定 李莉 韩连书 孔宪涛 |
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| 作者单位： | 第二军医大学长征医院儿科;长征医院临床实验科 |
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| 基金项目： | 国家自然科学基金资助项目 ( 3940 0 12 3) |
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| 摘 要： | 目的：探讨雷公藤红素诱导人肥大细胞系HMC-1细胞凋亡的基因调节机制。方法：雷公藤红素(0.5umol/L）与HMC-1细胞共育不同时间（4、8或12h）后,用RT-PCR、免疫组化方法检测bcl-2家庭、c-myc、白细胞介素1β转换酶（ICE）基因在mRNA和蛋白质水平表达的变化,并分析它们在细胞凋亡中的作用。结果：HMC-1细胞本已存在Bcl-2、Bax、C-myc蛋白表达;雷公藤红素作用后,Bcl-2蛋白表达受到抑制,Bax、C-myc蛋白表达增加。HMC-1细胞能自发表达bcl-2、bax、bcl-XL和ICE的mRNA,在雷公藤红素作用下,bcl-2、bax、bcl-XL的mRNA表达水平均下降（以bcl-2下降最为明显）,而ICE mRNA3次检测2次上升,1次下降。结论：雷公藤红素诱导HMC-1细胞凋亡可能与其上调促凋亡基因和下调抑凋亡基因的表达有关。
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| 关 键 词： | 雷公藤红素 肥大细胞 脱噬作用 基因表达 |
| 文章编号： | 0258-879(2001)09-0833-03 |
| 修稿时间： | 2000-12-16 |
Effect of tripterine on expression of bcl-2 family, c-myc and ICE gene in HMC-1 cells |
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| BAO Yi-Xiao,ZHANG Lu-Ding,LI Li,HAN Lian-Shu,KONG Xian-Tao. Effect of tripterine on expression of bcl-2 family, c-myc and ICE gene in HMC-1 cells[J]. Former Academic Journal of Second Military Medical University, 2001, 22(9): 833-835 |
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| Authors: | BAO Yi-Xiao ZHANG Lu-Ding LI Li HAN Lian-Shu KONG Xian-Tao |
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| Affiliation: | BAO Yi-Xiao1,ZHANG Lu-Ding1,LI Li2,HAN Lian-Shu1,KONG Xian-Tao2 |
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| Abstract: | Objective: To explore the molecular mechanism of HMC-1 cell apoptosis on exposure to tripterine. Methods: After the HMC-1 cells were incubated with tripterine, the expression of bcl-2, bax, bcl-X,c-myc and ICE were assayed by using immunohistochemical staining and RT-PCR. Results: The expression of bax,c-myc were up-regulated and bcl-2 down-regulated at protein level.The expression of bax,bcl-X L, especially bcl-2 were down-regulated, and ICE was up-regulated at mRNA level. Conclusion: These results suggest that apoptosis of HMC-1 cells induced by tripterine is regulated by different expression of apoptosis-related genes. |
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| Keywords: | tripterine mast cell apoptosis gene expression |
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