茯苓多糖对肝叶部分切除老年大鼠术后认知功能障碍的影响

目的 探讨茯苓多糖对肝叶部分切除老年大鼠术后认知功能障碍(postoperative cognitive dysfunction,POCD)的影响. 方法 将30只老年SD大鼠按照随机数字表法分成假手术组、模型对照组和茯苓多糖组,每组10只.建立肝叶部分切除老年大鼠POCD模型,采用Morris水迷宫实验评价肝叶部分切除老年大鼠的学习记忆能力,ELISA检测大鼠血清中TNF-α和IL-6水平. 结果 与模型对照组比较,术后第1、3、7天茯苓多糖组逃避潜伏期相对缩短、穿越平台次数相对增多;与模型对照组比较,术后第1、3、7天茯苓多糖组血清中TNF-α和IL-6水平分别为(18.9±3.9) ng/L和(14.7±3.8) ng/L,(8.9±2.2)ng/L和(9.9±2.6) ng/L,(6.6±1.8) ng/L和(9.6±2.7) ng/L,差异有统计学意义(P＜0.05);与模型对照组比较,茯苓多糖组脾脏系数和胸腺系数分别为(2.59±0.92) mg/g和(1.60±0.40) mg/g,差异有统计学意义(P＜0.05). 结论 茯苓多糖能够改善肝叶部分切除老年大鼠POCD的症状,其机制可能是抑制炎症反应和提高机体免疫力等综合方面.

Effects of pachyman on postoperative cognitive dysfunction in mice with hepatolobectomy

Objective To explore the effects of pachyman on postoperative cognitive dysfunction (POCD) in mice with hepatolobectomy.Methods Tirty SD aged mices were randomly divided into sham-operated group,model control group and pachyman group by random number table (n=10),to establish hepatolobectomy triggering postoperative cognitive dysfunction model in mices.Postoperative cognitive dysfunction was evaluated by Morris water maze,and enzyme-linked immunosorbent method was used to measure the content of TNF-α and IL-6 in serum.Results At the 1,3,7 day after surgery,compared with model control group,the time spent in target quadrant with the previously located hidden platform was relatively deacrease and the number of platform-site crossovers was relatively increased.The serum levels of TNF-α and IL-6 in three groups were (18.9±3.9) ng/L and (14.7±3.8) ng/L,(8.9±2.2) ng/L and (9.9±2.6) ng/L,(6.6±1.8) ng/L and (9.6±2.7) ng/L respectively (P＜0.05).The spleen coefficient and thymus coefficient in pachyman group were (2.59±0.92) mg/g and (1.60±0.40) mg/g (P＜0.05).Conclusions Pachyman can improve the symptoms of POCD in mice with hepatolobectomy,and the mechanisms may be inhibit inflammatory reaction and improve immunity.