依托咪酯乳剂对老年大鼠海马神经元凋亡及突触素表达的影响

目的观察腹腔注射依托咪酯乳剂对老年大鼠海马神经元突触素表达的影响。方法选择18月龄的sD大鼠45只，用完全随机分组法分为3组（每组15只）：生理盐水组（N组）、依托咪酯组（E组）、脂肪乳组（L组）。实验第1-3天，E组腹腔注射依托咪酯（乳剂）20mg／kg，L组腹腔注射20％脂肪乳20mg／kg，N组腹腔注射同等体积的生理盐水，每天1次，连续3d。实验第4天，处死大鼠，取右侧脑海马组织，固定，石蜡切片，进行细胞凋亡TUNEL检验、天冬氨酸特异性半胱氨酸蛋白酶（cysteinyl aspartate specific proteinase -3, caspase-3 ）Western blot检测及突触素免疫组化检验。结果TUNEL结果：海马CA1区神经元凋亡，与N组、L组比较，E组海马神经元凋亡细胞及凋亡指数无明显增加，差异无统计学意义（P〉0．05）。caspase-3表达结果：海马CA1区神经元easpase-3表达，与N组、L组比较，E组海马神经元活化caspase-3片段表达无明显增加，差异无统计学意义（P〉0．05）。突触素结果：海马CA1区神经元突触素表达，与N组、L组比较，E组海马含突触素的神经元个数无明显减少，差异无统计学意义（P〉0．05）。结论腹腔注射依托咪酯乳剂20mg／kg对老年大鼠海马神经元的凋亡及突触素的表达无明显影响。

Effects of etomidate emulsion on neuronal apoptosis and synaptophysin expression in aged rat hippocampus

Objective To investigate the effects of etomidate emulsion on neuronal apoptosis and synaptophysin expression in aged rat hippocampus.Methods Forty-five male and female SD rats aged 18 months were randomly divided into 3 groups (n=15):NS group(N group),Etomidate group(E group),Lipid group(L group).On the 1st day to 3rd day,animal received intraperitoneal (IP) injection of either etomidate 20 mg/kg,or lipid 20 mg/kg,or same volume NS depending group division once a day for 3 d.On the 4th day,all of the animals were killed and the brains were immediately removed for determination of neuronal apoptosis and synaptophysin expression in the hippocampus.Results The numbers of apoptosis cells and apoptosis index in hippocampus determined by TUNEL assay were not significant different among 3 groups (P＞0.05).No significant difference was found in the expression of synaptophysin among 3 groups (P＞0.05).The expression of activated caspase-3 was also not significantly different among 3 groups(P＞0.05).Conclusions Etomidate(20 mg/kg) does not affect the neuronal apoptosis and synaptophysin expression in aged rat hippocampus.